FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741459
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT1106494.036135503029591No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC612492.234175305922868No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN369971.3495368706954947No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA355501.2967547572296356No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA330401.205197670291622No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG308471.1252037692338277No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT294631.074719702173186No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA254200.9272434860415566No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT224240.8179586125490114No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210970.7695537303311849No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA193200.7047342309332367No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182910.6671994729813577No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAC159280.5810044943221839No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA150530.5490871831386134No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131400.47930682165956157No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC125790.4588432655750095No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA114540.4178067226247046No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC107440.3919081044071788No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAA106910.3899748272726311No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTG97160.3544098233823668No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT80980.29539015538806157No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA75470.27529136857417896No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC74370.27127890659681575No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC71710.2615760439970104No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA66010.24078419556885586No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA63630.23210268692692468No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC58810.2145208080806607No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA58610.21379126953932195No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA57790.21080016151983305No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG53940.19675654459906206No Hit
GGGCCGCCGGTGAAATACCACTACTCGCCTTGGCC53760.19609995991185714No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA51270.18701720507218966No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC46320.16896112617405548No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT45570.16622535664403518No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA43660.15925826357425008No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA43110.1572520325855685No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA42010.15323957060820534No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA40790.14878938550603893No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA38590.14076446155131264No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA37650.13733563040702051No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA37060.13518349171007116No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36750.1340527069709961No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA35930.1310615989515072No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC35840.13073330660790478No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC35720.13029558348310152No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC35360.12898241410869174No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC33120.12081158244569772No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT32710.11931602843595326No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT32420.11825819755101206No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGAGC32080.11701798203073618No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTT31850.11617901270819662No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG31550.1150847048961885No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC29880.10899305807600988No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA29760.10855533495120664No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG28630.10443344219264268No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGCGGCA28140.10264607276636274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC27930.10188005729795703No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA27540.10045745714234645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)