FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4797152
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT62307812.988498175584178No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2832595.904732641367212No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1619093.375106729993129No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC931331.9414227441615357No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA911151.899356117963325No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT564631.1770108597768008No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA544471.1349859249821561No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN524811.0940032752766642No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN491731.0250456937783086No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT431910.9003467057120558No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA423350.8825027849857583No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA338640.7059188451814744No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN328990.6858027429608234No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG311260.6488433136994617No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA310070.6463626751872777No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA303360.6323752092908459No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA298960.6232031005063003No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT252970.5273337180060169No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA251350.5239567143171615No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA236060.49208363629086593No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC232940.4855797773345518No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN228530.47638682284822326No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT228210.4757197603911655No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA191640.3994870289705225No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA168840.3519588289051504No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG144060.3003031798867328No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA142380.296801101987179No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC138460.2886295868882203No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA134760.2809166772284889No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACTG127580.2659494633482533No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT122600.25556830386029045No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC119960.25006503858956314No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA119250.24858499376296603No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC117090.2440823221778255No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT114090.2378286116429081No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG109300.22784352048882336No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC102360.21337660345138115No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT96770.20172385615465177No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA96120.20036888553875298No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA93510.19492815737337488No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG89210.18596450560665995No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACCA88780.18506814042998845No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA88050.18354640419982524No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC84160.1754374262062157No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA78930.16453512417367638No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC78560.16376383320770324No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT77540.16163757162583134No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA77480.161512497415133No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA77190.1609079720634243No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC76930.1603659838170648No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT75430.15723912854960612No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT74580.15546724389804617No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT74210.15469595293207303No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC70790.1475667229222672No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC68840.1435018110745709No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT67420.1405417214213767No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC66900.13945774492865767No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA66820.13929097931439322No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA65970.1375190946628333No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT64770.13501761044886632No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC60760.1266584840338601No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC60420.1259497301732361No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC59410.12384431429314727No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT57790.12046731060429189No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGAGAC55390.11546434217635797No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA54430.1134631548051844No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT53670.11187888146967201No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT52880.11023207102881043No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC52650.1097526198878001No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA52120.10864779769329802No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA50530.1053333311097918No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG50510.10529163970622568No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC50480.10522910260087652No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC50210.10466626865273396No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA50040.10431189172242197No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT49760.10372821207249636No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA48970.10208140163163476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)