FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4577738
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1594323.4827681269657638No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN650541.421094872620495No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA562651.229100485873154No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN438220.9572850171853435No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA420550.9186851672157733No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT405700.8862455649493265No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT318180.6950594376523951No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA259990.5679442554379477No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA252940.5525436361801396No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC247100.5397862437736717No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA245750.5368371890221765No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG226240.49421788665056854No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN209210.45701610708170715No Hit
CTGACCTATGAATTGACAGCCACGCCTTCCGGCAT205540.4489990471276425No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN197200.4307804422184057No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACGT196510.4292731475676415No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT158050.3452578544250457No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC150110.32791304351625195No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT149360.32627467976542124No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT106740.23317192901821815No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT105390.23022287426672303No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC103170.22537331756426426No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT101310.22131017546220427No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT99160.21661353270982306No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGCGT97600.2132057361080953No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA96510.2108246474568881No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA92770.2026546735527459No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA86570.18911086654587922No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT84470.1845234480435534No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA84270.18408655104333188No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC75000.16383637508306503No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT74630.16302811563265526No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT73890.1614115967318357No Hit
TATTGCACTTGTCCCGGCCTGTCGCACCAATTACG72900.1592489565807392No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC69110.15096975842654167No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCATAATG67560.14758380667482499No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC66680.14566145987385037No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG63230.13812498662002937No Hit
TCCCCGGCATCTCCACCACGCCTTGCGGCATGTCG60640.13246717046716086No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG59450.1298676333158429No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA59420.12980209876580967No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA58900.12866616656523375No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT55540.12132629696151244No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC54970.12008114051088113No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT54000.11796219005980683No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC53660.11721946515943027No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA53080.11595246385878791No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC52930.11562479110862178No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA51690.11291602970724841No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCGCGGCA50980.11136504535646209No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT50240.1097485264556425No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA50210.10968299190560929No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC50050.10933347430543205No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGAGT49900.10900580155526596No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGCGGCA49630.1084159906049669No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT48040.10494265945320592No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA47970.1047897455031284No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA47920.10468052125307302No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGCGGCAT47750.10430915880288476No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA47620.10402517575274076No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA47590.10395964120270755No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA47460.10367565815256356No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGCCGTA47390.10352274420248604No Hit
TGACCTATGAATTGACAGCCAGACGCCTGTTAATG47330.10339167510241958No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA46380.10131641435136743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)