FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4386987
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT51698711.78455737388782No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2894816.598629081873277No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC929732.1192905290122814No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA847491.9318270147597885No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA767791.750153351263635No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA712681.6245318256014891No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC688431.5692547071600622No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN518431.181745010869647No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN464671.0592007680898075No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA409970.934513824636362No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT364640.8311855038549236No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT351070.8002531122157417No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT333270.7596785675453335No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA321870.7336926232058586No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA270800.6172801515026144No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT257410.5867580642477399No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN238920.5446106861041531No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG234000.5333956995997481No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA215390.49097478519995613No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA213810.48737322449325704No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN207620.47326331261068244No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT172280.39270688515831026No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA158680.36170610945507703No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC155080.3535000217689271No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC153320.3494881566779204No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA149460.3406894071033263No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA148870.3393445205103184No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT120370.2743796596616311No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT120150.27387817652525526No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT119090.2714619395954444No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC115990.26439558631014864No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA114640.2613183034278424No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA113840.25949472838647575No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC108650.24766428530560952No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG104410.2379993375863662No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT103840.2367000403693925No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA103790.23658606692930706No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC100720.2295880977080625No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA100690.22951971364401125No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA97020.2211540631417417No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG94260.21486272924902675No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA93010.2120133932468913No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA89220.20337420648841678No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC87690.19988661922180304No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG87680.19986382453378596No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG86790.19783509730026552No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT84710.19309380219271222No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA83910.19127022715134556No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA83360.19001651931040597No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC79510.18124056442382894No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA74060.16881745945451856No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA72240.1646688262354094No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT71790.16364306527464065No Hit
AACCCGTAGATCCGATGAACACGCGGAACCGGCAT71640.16330114495438441No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA70730.16122682834482982No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA69550.15853705515881403No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT69340.15805836671045526No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT68470.15607522885296904No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA68220.15550536165254192No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC67380.15359060785910694No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA66080.15062729841688613No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC64690.14745883678251154No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA63570.14490583172459823No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC61720.14068881444143783No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA61260.139640258792652No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC59150.13483057962104741No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC58960.13439748054872286No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT57820.13179888611477536No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA57080.1301120792015112No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT54640.12455017532534288No Hit
AACCCGTAGATCCGAACTTGTGACGCCTTGGCCGT53390.12170083932320748No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC53320.1215412765070879No Hit
AACCCGTAGATCCGAACTTGTCGCCTTGGCCGTAC52590.11987726428184081No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT52310.11923901301736249No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC52190.11896547676115748No Hit
TCCCTGAGACCCTAACTTGTGCGCCTTGGCCGTAC52160.11889709269710623No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT51780.11803089455245706No Hit
TCCCTGAGACCCTTTAACCTGCGCCTTGGCCGTAC51060.11638967701522707No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT50020.11401902946145041No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA46250.10542543207901003No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA46230.10537984270297587No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT45790.1043768764302242No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC44760.10202902356446462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)