FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845278
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC573272.0148119094162324No Hit
CGCCTTGGCCGTACAGCAGACTCACGATAAGAGAA491511.7274586174004791No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT396731.3943452977178328No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA390081.3709732405761406No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN383791.3488664376556527No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT372251.308308010675934No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA307031.0790861209344043No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA268070.9421574974396175No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN254790.8954836750574109No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT203810.7163096189546329No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA199840.7023566765707956No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA172050.6046860798839341No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT170140.597973203321433No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA160280.5633192960406681No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149760.5263457560210285No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA143180.5032197205334593No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143170.5031845745828702No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT117830.41412473579031644No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA110310.3876949809473802No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC103730.364568945459811No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA100250.35233815465483515No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC93600.3289660975131428No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA84400.29663182297125273No Hit
CGCCTTGGCCGTACAGCAGACTCACGATAAGAGCC78410.2755793985684351No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG71310.25062577365023736No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA69270.24345599973007911No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC65390.22981937090154284No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC62730.2204705480448659No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG61140.21488234190121316No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA60670.21323048222352967No Hit
CGCCTTGGCCGTACAGCAGACTCACGATAAGAGTT58050.20402224316920878No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC57850.20331932415742857No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC56810.1996641452961714No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT56400.19822316132202195No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC55310.19439225270781976No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT55170.19390020939957361No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC55130.19375962559721757No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA54100.19013959268654942No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA49140.1727072011944No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC48890.1718285524296747No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT48230.16950891969079998No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA48110.16908716828373185No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA46070.1619173943635736No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA45470.15980863732823297No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA44050.1548179123445934No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC42480.14929999810211866No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT42460.14922970620094064No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT41910.14729667891854503No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG41480.14578540304321758No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT41110.14448500287142416No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG40080.14086496996075604No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA39390.13843989937011428No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC39140.137561250605389No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC37290.13105924974642197No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG35650.12529531384982417No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT35030.12311626491330549No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC34150.12002342126147252No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT33960.1193556482002813No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG33620.11816068588025494No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC32900.11563017743784615No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC32560.11443521511781977No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA31360.11021770104713846No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT31100.10930390633182416No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT30840.10839011161650988No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA30240.10628135458116922No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA30220.10621106267999118No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG29860.1049458084587868No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG29720.10445376515054065No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT29680.1043131813481846No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA28670.1007634403386945No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC28600.10051741868457142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)