FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827355
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT50110417.72341994549676No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1918746.786342712535213No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC467201.6524277991267458No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG318881.1278385628971246No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC229700.8124200887401829No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA225480.7974944780545775No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA221870.7847263608567018No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT216810.7668297755322554No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA215060.7606402450346702No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206250.7294803800725413No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA178470.6312260045165888No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA178380.6309076858052844No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA162290.5739993739732011No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG159580.5644144438883691No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT146300.5174447495981226No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT145230.5136602938081706No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT115650.4090395440261304No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG114890.40635151935289343No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG101080.3575072815405211No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA97450.3446684268512444No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG95830.3389386900477655No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG79480.2811107908274695No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA78790.2786703473741359No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA67780.23972935835789988No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA60640.21447607392775228No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60540.21412238647074738No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC57810.20446671889451448No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC53630.189682583191711No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC53290.18848004583789443No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT53040.18759582719538226No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA50480.17854142829605762No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAT50290.17786942212774837No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA49020.17337759142378653No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC48350.17100788546185391No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA47280.16722342967190185No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC44280.15661280596175578No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG44140.15611764352194896No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC43590.15417236250842217No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC43580.1541369937627217No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA42850.15155507532658616No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA42450.15014032549856668No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA42380.14989274427866328No Hit
GNNTGACCCCTTTGAAGGCTTTCTATTCCAATTAC39220.13871622063730943No Hit
GNNGTCAAAAGGGTCCTTAGGGAGCGGAACCGGCA38690.13684167711518364No Hit
GNNACTGGGGAAACTTCCGAAAGATAAGGTTAATG38200.13510860857585977No Hit
GNNCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT37940.1341890211876471No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC35650.12608957842223562No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA35640.12605420967653513No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA31680.11204818637914234No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC31670.11201281763344186No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGTCG31550.11158839268503601No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31250.1105273303140214No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT29970.1060001308643591No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA29470.10423169357933475No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGTCT29400.10398411235943135No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA29360.1038426373766294No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG29280.1035596874110255No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA29080.10285231249701576No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT28770.10175588138030067No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGCGGCATG28720.10157903765179822No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC28310.10012891907807828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)