FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213541
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC544291.6937390871938462No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA364821.135258582355103No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG341581.062939604629286No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT284620.8856896488950973No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA270400.8414393966033109No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAG234930.7310627124408868No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT225360.7012824793584398No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG200520.6239845702917748No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC193130.6009881311612331No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA189590.5899722455696069No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAC184750.5749109782635418No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC168900.5255884396682662No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA156660.4874996149107791No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT130390.40575178595823114No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC125770.39137512171153255No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA116230.36168824359172635No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA113940.3545621481101377No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGAGC106330.3308811059202294No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG104960.3266178959596283No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101870.31700233480761564No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAT98340.30601756753686976No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA95150.29609082317605406No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA93250.29017834220879707No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC88400.27508595658185164No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT83300.25921561293289863No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA77110.23995337230799293No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC73780.22959097145485308No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA72140.22448756683048388No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT70570.2196019904522768No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC69790.21717476142361342No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGCTCA66730.20765255523424161No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA65210.202922570460436No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC63890.19881495210423641No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACATA62770.19532970016564283No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC61750.19215563143585224No Hit
GCATTGGTGGTTCAGTGACGAGGCCTCTATTCTAT60030.18680328024444065No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGAT59650.18562078405098922No Hit
GCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59170.18412710464873486No Hit
GCCCGGCTAGCTCAGTCGGATCTCGTACCCTGAGC57540.17905481834524595No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC57230.17809015039795664No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA57110.17771673054739306No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA55250.17192872286365724No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC52100.16212645178636276No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG51910.16153520368963709No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT51250.15948139451153728No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC50670.1576765319004799No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC49780.15490700134213317No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC48780.1517951692541032No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA48530.1510172112320957No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAA43960.1367961385897986No Hit
TAGCTTATCAGACTGATGTTCTGTATTGTGGCTTG42660.13275075687535962No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG41880.1303235278466962No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG39590.12319743236510752No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC39350.12245059266398031No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTAGGT39250.12213940945517733No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA39040.12148592471669102No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG38000.11824961934513982No Hit
GCCCGGCTAGCTCAGTCGGATCTCGTACTCTGAGC37450.11653811169672333No Hit
TAGCTTATCAGACTGATGTTCTGTATTGTGGCAAC37440.11650699337584303No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA37400.11638252009232185No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA37300.11607133688351884No Hit
GCCCGGCTAGCTCAGTCGGATCTCGTACCCACTCG36880.11476436740654623No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36720.11426647427246142No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA36700.11420423763070084No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA36010.11205707348996015No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35810.11143470707235414No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG34720.10804281009640146No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGCTCT34440.10717149711175304No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG34010.10583340931390015No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTACCA33560.10443308487428665No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA33450.10409078334460335No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA32580.10138348942801727No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG32550.10129013446537635No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)