FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014072
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2611856.506734308701986No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1481943.691862029380639No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT902582.2485396375550812No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT748301.864191773341385No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN627791.563972943185872No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN602671.5013930990774456No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA518151.2908338465279148No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA483811.2052848080452967No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA453451.129650888175399No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA450331.1218782323784924No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA423971.056209255837962No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT362260.9024750926241483No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA321260.8003344234981336No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC304860.7594781558477277No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN296480.7386015995727032No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT274330.6834207258863318No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA257810.642265509936045No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN242090.6031032826516316No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA227740.5673540484575263No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA196830.49034994887984074No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCA178890.44565717804762844No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC172140.42884133617932113No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA163020.40612126538836374No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAA158840.3957078996091749No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAT151440.3772727544498455No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC147760.36810500658682754No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCG129710.3231381998130577No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT128150.3192518719146044No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC122900.30617288379480984No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT118480.295161621415859No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC108340.26990049007591294No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT105070.26175414890415516No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA101480.252810612266048No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC101410.2526362257577841No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT101060.25176429321646443No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC100200.2496218304006505No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA99840.24872498550100744No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT99230.24720533164327893No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA97700.24339374081979595No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA95280.23736495010552874No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAC91980.22914387185880075No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG88810.22124665427027718No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA87300.217484888163441No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC86580.2156911983641549No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT84740.21110732443264593No Hit
CCACTACGCCTTGGCCGTACAGCAGTATAACCTAT80460.20044483507022295No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT79450.1979286868795577No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA77670.19349428709798927No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA75770.1887609390165398No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA75330.1876647952503094No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC73010.18188512811927637No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC72710.18113775736957383No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCC72350.1802409124699308No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC71790.17884582040381936No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA68190.16987737140738882No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA67540.16825806811636662No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC65650.16354963239324058No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC62430.15552785301309993No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA61330.15278749359752392No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT60810.15149205096470617No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG58910.1467587028832567No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA58110.14476571421738324No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA57700.14374430752612308No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA56420.14055552566072554No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA56320.14030640207749137No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA56050.1396337684027591No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC55310.13779025388682614No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA54070.1347011214547223No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC53690.1337544518384324No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT53380.1329821687304064No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG53040.1321351485474102No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA51820.1290958408319532No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG51360.12794987234907595No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA51090.12727723867434368No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA50210.12508495114188287No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC50080.12476109048367842No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC49400.12306705011768598No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA48760.12147265918498723No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT48450.12070037607696125No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC47950.11945475816079033No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGATGC45560.1135007045214934No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC45470.11327649329658263No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44290.11033683501441927No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA44210.11013753614783192No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA44160.11001297435621483No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA43710.10889191823166101No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA43670.10879226879836734No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG42280.10532945099141222No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG41840.10423330722518181No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA41030.10221540620098495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)