FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3531633
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA874342.475738560603551No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT830202.3507538863749433No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA475741.3470822138087395No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN475141.3453832830308246No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA461111.3056566183405807No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA371021.0505621620366556No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA361011.0222183335584418No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA339090.9601507291386165No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN330560.9359975965792595No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT310750.8799045653951019No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT296510.8395832749325878No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA239700.6787228457770103No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC232540.6584489384938922No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA193530.5479901224164572No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN191230.54147755443445No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT189080.5353897191469216No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT160810.4553417639941636No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN159180.45072633538082807No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA157800.44681879459162377No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA156100.4420051573875315No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT155210.43948507673362436No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC152190.43093379181811925No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA152100.430678952201432No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA133860.3790314565528185No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA107800.3052412297653805No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT105640.2991250789648868No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT104530.29598205702574415No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA95530.27049809535702035No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC95380.27007336266254167No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC91210.258265793756033No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT90390.2559439216928826No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA90330.2557740286150911No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA84380.23892629840076815No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC78490.22224846126423667No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT77390.21913375483805933No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT75380.21344233673204435No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA74540.21106383364296347No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA72860.2063068274648017No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT71950.20373011578496406No Hit
TCGAGTCCACTTAAGCCCACCACGCCTTGGCCGTA68750.19466915163608448No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC67380.19078992635984543No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTCGGCA65770.18623112877244039No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG65050.1841924118389425No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC64660.18308810683329782No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA63550.1799450848941552No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAGCCGT61920.17532965628081967No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA60420.17108232933603237No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC58570.16584395943746136No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT58250.1649378630225734No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC57020.16145505492784784No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC56890.1610869532592996No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG55270.15649984015892932No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT54960.1556220592570066No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT54070.15310197860309946No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT53670.15196935808448953No Hit
CAACGGAATCCCAAAAGCAGCTCGCGAACCGGCAT49390.13985031853536312No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA49260.13948221686681486No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC49020.1388026445556489No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48550.1374718154462822No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT47840.13546141402574957No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC46390.13135566464578852No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA45920.13002483553642183No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC44430.12580582410459976No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44410.12574919307866927No Hit
TCCCTGAGACCCTAACTTGTGACGCAGGTTAATGC43990.12455994153412883No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC43450.12303090383400539No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC42670.12082229382271602No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA42330.11985956638189754No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAATTAC40310.11413983276291732No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT39730.1124975330109329No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG38280.10839178363097185No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG37940.10742905619015339No Hit
TCGATTCCCATCACCCGCTCCACGCCTTGGCCGTA37840.10714590106050091No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA37370.10581507195113421No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA37240.105446970282586No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC37150.10519213066589875No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA36620.10369140847874057No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA36450.10321004475833134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)