FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718223
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2073877.62950648272787No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1016883.7409734227103515No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT646702.3791278346184255No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA584372.149823616384675No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT516041.8984461539763293No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA445971.6406674507573513No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA390071.4350183925307085No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA338081.2437537317578433No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC285611.0507232114510106No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT260380.9579052196968387No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA216870.7978374106907344No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT199680.7345975661305199No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC142320.5235773518213921No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGAC136700.5029020797778548No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC124660.45860843646750105No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA124330.45739440803789827No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT122100.44919051895300716No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA117740.4331506281861348No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA114600.42159896373476347No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC107920.3970240852203811No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT101870.3747668973443312No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT99090.36453962754343555No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA90750.3338578181407486No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT90700.33367387443929364No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA79970.29419955610705967No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA79370.29199223168960015No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA78420.28849730136195595No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA73050.26874174782569343No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT73010.2685945928645295No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGCA70560.2595813514932366No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67990.25012664523845174No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA66910.24615346128702464No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT66380.2442036580516021No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA65610.24137092504919574No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC63230.23261520485993978No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATAC58380.21477266581880883No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATCA56810.20899683359312313No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG56330.20723097405915555No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT52120.19174291439664812No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA50900.18725468808114715No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT48680.17908758773654698No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA48570.1786829115933461No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT46890.1725024032244595No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA46680.17172983967834868No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT45650.16794059942837655No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA44720.16451924658131434No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA44360.16319485193083866No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT43690.1607300063313422No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA43290.15925845671970254No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA43000.15819158325126378No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG42900.15782369584835387No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC42020.15458628670274663No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA41980.15443913174158264No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA41970.15440234300129166No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC41370.15219501858383216No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA41180.15149603251830332No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41070.1510913563751024No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA39940.14693422872222037No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA38730.14248279114701037No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC38250.14071693161304277No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37910.13946611444314908No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC36970.13600797285579586No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGGCGT36810.13541935301114No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC36110.1328441411907706No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC35260.1297170982660363No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC35130.1292388446422534No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA34270.12607501297722815No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT33730.1240884210015146No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA33640.12375732233889566No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT33020.12147642044085419No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32960.12125568799910824No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC32270.11871726491902983No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC31430.11562701073458653No Hit
CTGACCTATGAATTGACAGCCGCCTTGTAATGCAC30590.11253675655014322No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG30080.11066053079530266No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT29690.10922576992395398No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG28330.10422250124437914No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA28100.10337636021768633No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC27970.10289810659390344No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG27510.10120582454051782No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG27270.10032289477353404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)