FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192800
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2110855.034463842778096No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1302593.106730585766075No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA874892.086648540354894No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN702321.6750620110665904No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA644661.537540545697386No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN523831.2493560389238694No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT473821.1300801373783629No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT468101.1164377027284869No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA421031.0041738217897347No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA383310.91421007441328No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA364030.8682264834955161No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA346890.8273468803663423No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN323080.7705590536157222No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA293360.6996756344209121No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286390.683051898492654No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC280830.669791070406411No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC251240.5992177065445525No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA250190.5967134134707117No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT231860.5529956115245183No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC208310.4968278954398016No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC207260.4943236023659607No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA205160.48931501621827894No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA204820.48850410227055907No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA178850.4265645869108949No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA178030.42460885327227627No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT169510.40428830375882463No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAC163890.39088437321121927No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC146910.3503863766456783No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA143840.34306430070597216No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA137660.32832474718565163No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA126110.300777523373402No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC115790.2761639000190803No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA112480.2682694142339248No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAG111840.2667429879793932No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA104980.2503816065636329No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC99550.23743083381034152No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA95640.2281053234115627No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT91200.21751574127074985No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA90200.2151307002480443No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT89790.21415283342873498No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG86660.20668765502766645No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC85120.20301469185269988No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT84920.20253768364815872No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA83860.20000954016409084No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC83400.19891242129364625No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA81150.19354607899255866No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA79590.18982541499713795No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA77210.18414901736309863No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT74870.17856802136996755No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA74120.17677924060293837No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT73610.1755628696813585No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT72600.17315397824842588No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC71400.17029192902117918No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA71230.16988647204731921No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC70380.16785918717801945No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA70140.1672867773325701No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA68800.16409082236214464No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC68780.1640431215416905No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC68370.16306525472238123No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC65490.15619633657698911No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT64980.15497996565540928No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA64940.15488456401450104No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG63940.15249952299179545No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA60980.1454398015645869No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC60530.1443665331043694No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC60390.1440326273611906No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC59460.14181453921007442No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA59430.14174298797939325No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG59270.14136138141576035No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA58690.1399780576225911No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT56920.1357565350124022No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC55280.13184506773516505No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA53730.12814825414997139No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA52290.12471379507727533No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT50630.12075462697958406No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT49660.11844113718755964No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG46780.11157221904216752No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC45160.10770845258538447No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA45040.10742224766265979No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43330.10334382751383323No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43330.10334382751383323No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC43250.10315302423201679No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT43050.10267601602747568No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA42580.10155504674680405No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA42520.10141194428544172No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA42470.10129269223430643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)