FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350895
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC932432.7826297153447066No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN682172.0357844695223215No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT567291.6929506892934574No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA528931.5784738107281786No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT458241.3675152459268345No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT376231.1227746616948606No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA369171.1017056637107399No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA363761.0855607233291404No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN331320.9887507665862404No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA284580.849265643954824No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN280750.8378358617623052No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA273800.8170951342850193No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT269270.8035763579580978No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC244830.730640619894088No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC231370.690472246966855No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA231190.6899350770465801No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA203380.6069423243640878No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172840.5158024945574242No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC156180.4660844341586352No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA146060.43588354752983904No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA120370.3592174627972527No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA114230.3408939999612044No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA105280.31418471781419594No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC93240.2782540187024661No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC92990.27750794936875073No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC89250.26634675213636955No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG88000.2626164054677929No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT86940.25945307149283997No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT86680.25867715938577607No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT84910.25339498850307157No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC83090.24796360375362406No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA79660.23772753249504983No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC78400.2339673430531246No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA72500.21636010677744305No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC70680.21092872202799548No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA69960.20878004234689537No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT69750.20815334410657452No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA69160.20639262047900636No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA67370.20105076404960467No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC66860.1995287826088254No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA64280.1918293470848833No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGACTT62850.18756183049603167No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGT60660.18102626313268544No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG57860.17267028659507386No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC57770.17240170163493634No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA57740.17231217331489052No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA55050.16428446728411364No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA51950.15503320754604366No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC51500.1536902827453561No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA50990.15216830130457681No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA49410.147453143115496No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA48110.14357358258017633No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT48040.14336468316673603No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC47770.14255892828632352No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA46860.13984323591159975No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT45300.13518776326921614No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC43370.12942810801293386No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA43120.12868203867921854No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGAGAA43060.12850298203912686No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA42760.12760769883866846No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA42660.12730927110518236No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA42420.12659304454481565No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT41440.12366845275665159No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC41320.12331033947646823No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA40930.12214647131587233No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA40270.12017684827486388No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39720.11853549574069018No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC39600.11817738246050681No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA39490.11784911195367208No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT39380.11752084144683733No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG39210.11701351429991091No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT39080.11662555824637896No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG37410.11164181509716062No Hit
GCTAAACCTAGCCCCAAACCCACACGCCTTGGCCG36950.11026904752312443No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG36610.10925439322927158No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC36400.10862769498895071No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT36380.10856800944225348No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA35480.10588215984087834No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA34480.10289788250601704No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA34260.10224134149234756No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC34010.10149527215863226No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT33700.10057014618482525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)