FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3923715
Sequences flagged as poor quality0
Sequence length35
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT81122220.674845140383542No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA39480810.06209676288925No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC1436403.6608163436946874No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN878192.238159499352017No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT578361.4740112367998186No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA529691.3499706273263987No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG512951.3073069782081523No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN488301.2444838628697548No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA445091.134358637158917No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA439071.1190160345488906No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA313070.7978917938739181No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT297750.7588471639759768No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC277850.708129922790009No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA247210.6300406629941268No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN224960.5733341998590621No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA208270.5307979809950519No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT194420.49549980057165216No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA184250.4695804868600294No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA170750.4351743182162823No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG156510.3988821818098409No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA154390.39347913903023024No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG150290.38302985818287005No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG145690.37130627479314887No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA140000.35680471186108065No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN138930.3540777044204281No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA136320.34742584514930364No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC127900.3259665903359444No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA112690.2872023069973227No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA111090.28312453886176747No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA107530.27405150476015716No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC103100.26276118423483863No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC92660.23615374715034096No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG88770.22623967337077233No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA86280.21989364670981457No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC85970.21910357913355072No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT84940.21647851589628705No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA84470.21528067150646774No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC84090.21431220157427333No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT81220.20699770498112122No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC79260.2020024390150661No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC75520.1924706559982058No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA70860.18059415630340123No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT70690.1801608934389985No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA70500.17967665847290132No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC69030.17593020899835998No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC68990.1758282647949711No Hit
TGAGATGAAGCACTGTAGCTCGCCTTGGCCGTACA68820.17539500193056837No Hit
TGGAATGTAAAGAAGTATGTATCGCCTTGGCCGTA68710.17511465537124893No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG62940.16040920403240297No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGC58000.14781909491387626No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC56900.14501562932068204No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG56090.14295125920205723No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA54610.13917932367666866No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG52590.1340311414055302No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA50680.12916330569371118No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC49040.1249835933547671No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC48980.12483067704968379No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA47330.12062547865989248No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA46870.11945312032092036No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC46580.11871402484635096No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGAGAG44570.11359132862605974No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA44100.1123934842362404No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT41480.10571613891426875No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC40840.10408503166004666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)