FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738971
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC972023.54885101010562No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA654872.390934405658183No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN488961.785195973232283No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN328041.1976760615574245No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG296281.0817201058353665No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA278451.016622665957398No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGAGAT278371.0163305854643951No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN255860.9341464367457706No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC254970.930897041261116No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC249200.9098307357032989No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT233950.854152891724666No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA222940.8139553138751744No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC217520.7941668604742438No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA214360.7826296810006385No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT206770.7549185442270108No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG197220.7200514353748177No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA195630.7142463355763898No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT170970.6242125236083186No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC166210.6068337342746601No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN160300.5852562878540882No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT160230.585000717422711No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC145460.531075356402094No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA135680.49536851613251837No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT135670.49533200607089306No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT133030.4856933498018051No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA124540.45469630748189743No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT118040.43096476742543094No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC117440.428774163727911No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA110400.4030710803436765No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG110150.40215832880304314No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC106520.38890517643304734No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA104730.38236987540211265No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT99370.3628004823709342No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT95960.3503505513566956No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC94220.3439978006338877No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC92220.3366957883088211No Hit
CCACTACGCCTTGGCCGTACAGCAGGTAGTAAGCT87230.31847726755777994No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG82040.2995285455742321No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGAGAA81340.29697284126045875No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG78750.2875167352994975No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA77530.28306250778120684No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG77140.2816386153778189No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC74020.27024747615071504No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA71280.26024371926537376No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC70120.2560085521168351No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA69730.2545846597134471No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA69210.25268613650892985No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA67080.2449094933827339No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC66560.24301097017821655No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT65960.24082036648069657No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG64280.23468667612764066No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGATCG58280.21278063915244083No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA55520.20270386214384895No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGCGCCTTGG53570.19558440012690897No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA53380.19489070895602764No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT50240.18342654960567306No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA49380.18028668430589445No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC48680.17773097999212112No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACGCC48120.17568641654110248No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA48030.17535782598647448No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG46510.16980829661942387No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG44490.1624332641711066No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT44070.16089984158284262No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG43860.16013313028871062No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA43520.1588917881934493No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA42360.15465662104491065No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA42000.15334225882639868No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA40710.1486324608767307No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCTTG40370.1473911187814694No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC38780.14158601898304143No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGG36950.1349046777056055No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCGCCT36860.1345760871509775No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36510.13329823499409085No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA36190.1321299130220802No Hit
CGCCTTGGCCGTACAGCAGGTAGTAATAGCTCTCG35710.13037743006406421No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA35550.12979326907805885No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA34940.12756615531891355No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC33860.12362306866337759No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC33850.12358655860175226No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT33250.12139595490423229No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTG31840.11624803621506033No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA30590.11168427851189369No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT29890.10912857419812039No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA29580.10799676228773507No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC29010.10591568877509108No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA28940.10566011834371375No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC28850.10533152778908576No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA28590.1043822661868271No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC27930.10197260211955513No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG27690.10109636064054714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)