FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3691458
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1834344.969147691779238No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1764824.780821019770507No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA849882.302288147393252No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA846312.292617171860008No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA642841.7414257455997062No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT603531.6349366564647356No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA560001.5170157699207198No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT520811.4108517555935893No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA411921.1158734570459694No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA373961.0130414594992005No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA351770.952929709616092No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC267100.7235623431175432No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT245880.666078281264476No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA238450.6459507327457065No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT195470.5295197724042913No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA192300.5209323795638471No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC179180.48539086723999025No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA174600.47298384540742433No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT161310.43698181043912726No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA135900.3681472198789747No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT135380.36673856237833397No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA135010.36573624838749347No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA120840.3273503314950353No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC109370.2962785977789806No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGAT95560.25886790531004283No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC95080.2575676060786822No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG94290.25542753026040116No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT93270.2526643943937599No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGCG93050.2520684239127196No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC92350.2501721542003187No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA91750.24854678016111792No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT89810.2432914041010354No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT86610.2346227425586313No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG85250.23093856140310956No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA84560.22906938125802867No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA78040.21140698336538027No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGACGC77150.20899601187389913No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG74310.20130257475501548No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA73230.1983769014844541No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA71300.19314861499169164No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA70780.19173995749105097No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA66320.17965801046632524No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC65430.1772470389748441No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA62920.17044755757752086No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61680.16708845122983926No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT58570.1586635957933153No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC57430.15557538511883381No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC56290.15248717444435234No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56220.15229754747311225No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT54980.14893844112543064No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54770.14836956021171038No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54730.14826120194243034No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT54490.14761105232675No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC53960.14617530525878936No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA52650.14262657193986766No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49730.13471641828242392No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC48180.1305175353478219No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC48150.1304362666458619No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA48100.1303008188092618No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC46000.12461200967205913No Hit
AAACCGTTACCATATGACTCAAGCGGAACCGGCAT45980.1245578305374191No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA44850.12149670943025763No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA44310.12003387279497695No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43760.11854394659237624No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGACTA43510.11786670740937591No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC41970.11369491404209393No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA41820.11328857053229374No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC41650.11282804788785353No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC41210.11163610692577294No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA41040.11117558428133274No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC40770.11044416596369239No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA39820.10787065706829117No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA39740.10765394052973107No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG38410.10405102807616935No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC37640.10196513139252837No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT37500.10158587745004818No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA37360.10120662350756801No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG37190.10074610086312778No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA37040.1003397573533276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)