FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071093
Sequences flagged as poor quality0
Sequence length35
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA636742.0733335004833786No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT540521.7600248510872187No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT460971.5009965507394272No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT332251.0818623858020582No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT325141.0587110191713505No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA290940.9473500151249084No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC260280.8475158518481857No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT225740.735047750100697No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA193520.6301339620779963No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC141830.4618225498218387No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA139710.4549194700388428No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA138260.4501980239608504No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133120.43346131165679447No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAC131420.427925823151562No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA120460.39223820314135716No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC119400.3887866632498592No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC115070.3746874484100612No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA114650.37331985713229787No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG111460.3629326757607145No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA100350.32675663029416563No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA95030.3094338074424969No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC92660.301716685232261No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA90680.29526946920851954No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC85870.27960729290842057No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA82660.269154988142658No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT81560.26557320146280167No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC80950.2635869379403359No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA79620.2592562322274187No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA74050.24111936694850988No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA65370.21285581387473448No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT63390.20640859785099314No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTTAATG59510.19377465938022717No Hit
TACCCTGTAGATCCGATAAACAGCGGAACCGGCAT58810.19149534058395498No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT55980.18228038030759733No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC55680.18130352939490924No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA55250.1799033764200563No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA51800.1686695909241433No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT51080.1663251487336919No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA50500.16443657030249492No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG49830.16225493659749152No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC49110.15991049440704008No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC48000.15629614603009417No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG46870.15261667425896902No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT46230.15053272564523446No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA46070.1500117384918008No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC43470.14154569724850402No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC42500.13838721263081255No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC42490.13835465093372296No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA41550.13529385140730027No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG39150.12747904410579555No Hit
TAGCACCATTGTCCCGACTGTGGATAAGGTTAATG37120.12086901959660616No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG37000.12047827923153093No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA36360.11839433061779635No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC36270.11810127534398991No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC36190.11784078176727307No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT34340.11181686780569652No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC34130.11113307216681488No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA33780.10999341276867877No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA33530.10917937034143871No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA33100.10777921736658577No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC32490.10579295384411998No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA31790.10351363504784779No Hit
TAGCACCATCTGAATAGCCAATGCGGAACCGGCAT31540.10269959262060771No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA30790.10025746533888749No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)