FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780685
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT40359510.675181878416213No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1842934.8745928317222935No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC927792.454026188375916No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA686191.8149885536615717No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA439931.1636251102644097No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT417641.1046675404060375No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC390951.0340718679286955No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA359440.9507271830369365No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA307920.8144555814620896No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA303910.8038490379388921No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA303460.8026587774437701No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC216540.5727533502526658No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA214500.5673575026747799No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT193660.5122352166340226No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG174920.46266747957050114No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN173060.45774773619066383No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA147750.39080219589836235No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT144310.3817033156689859No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG140330.3711761228454632No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA135590.3586387122968457No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT135140.3574484518017238No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA129330.3420808662980386No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG117260.3101554347955463No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA110090.29119061757327047No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA107380.28402260436931404No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT98580.2607463991313743No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA95510.2526261775313204No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT93360.24693937738796012No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT90120.23836950182308234No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA89730.23733794272731No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA89020.23545997616833989No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC86180.22794810993245931No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA81540.21567520171609111No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAC77320.20451320329517006No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA75340.1992760571166336No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC75250.19903800501760924No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT68730.18179245295495391No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT67140.17758686587218983No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC66770.17660820724286738No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG66660.17631725467739312No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC65630.17359288065522518No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC65470.17316967692362628No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA65370.17290517459137697No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC64030.16936084333923615No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC60060.15886010074893836No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG59260.15674408209094384No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58330.1542842104010252No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT56410.14920576562183838No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC56280.14886191258991424No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG54410.14391571897685207No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54340.14373056734427755No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52010.13756766300286852No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT51700.13674770577289566No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC50090.13248921822368168No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA48250.1276223753102943No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA47300.1251096031539258No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT46560.12315228589528089No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA46490.12296713426270635No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC46450.12286133332980664No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG44990.11899959927896664No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGCACA44860.11865574624704255No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC44770.11841769414801814No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAG44500.117703537850945No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC44160.11680422992129734No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA43770.115772670825525No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG43160.1141592065988042No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA42940.11357730146785569No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40970.1083666055225442No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC40790.10789050132449542No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC40760.10781115062482065No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT40400.1068589422287231No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC40200.10632993756422447No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA40150.10619768639809982No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT39820.10532482870167707No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC39790.10524547800200228No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC39700.1050074259029779No Hit
CTGACCTATGAATTGACAGCCCGCCTTAATTACGT39170.10360556354205652No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39140.10352621284238173No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)