FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435194
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC892172.597145896272525No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA679901.9792186409268295No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA365351.0635498315378986No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT360031.0480630788246603No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA310430.9036753091674007No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT278350.8102890258890765No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA255730.7444412164203826No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA252060.7337576858832427No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA249760.727062285274136No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC206760.6018874043212697No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC182780.5320805753619737No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTC174850.5089959984792707No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT169570.49362568751575603No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA160110.4660872137061255No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA158110.4602651262199457No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA154650.4501929148688546No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT141650.41234934620868574No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA128750.3747968819228259No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC123300.3589316935229859No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA120370.35040233535573245No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC118090.34376515562148746No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117250.3413198788772919No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC98080.28551517032225837No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTG96490.2808866107707454No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC86530.2518926150895699No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT78750.2292446947683304No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG75640.22019134872732077No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC74740.21757140935853986No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATCGT74490.21684364842276738No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG72700.21163288012263645No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC69490.20228842970731786No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67890.19763075971837402No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT66810.1944868324758369No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA65870.19175045135733237No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA65600.1909644695466981No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC63490.18482216724877837No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC63060.18357041843924973No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT62990.18336664537723343No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT62390.18162001913137948No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC62190.1810378103827615No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC61840.18001894507268001No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT61260.17833053970168788No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTAGGT59700.17378931146246762No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAGCA59290.17259578352780075No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT59190.17230467915349176No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC58700.1708782677193777No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGTCAG58660.1707618259696541No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC58400.17000495459645074No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTACCA57720.1680254448511496No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC56370.16409553579797823No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATCCA54610.15897209881013996No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54290.1580405648123512No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA52910.15402332444688713No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC52900.15399421400945623No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG49880.1452028619053247No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA49350.14366000872148707No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC49050.14278669559856008No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA48900.1423500390370966No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC48330.14069074410353535No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCAAC47440.13809991517218534No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG46350.13492687749221732No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC46300.13478132530506282No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC46100.13419911655644484No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCTTG45540.1325689320603145No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT45080.13122985193849315No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT44570.12974521962951727No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC43640.12703794894844367No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC43160.12564064795176053No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT42570.12392313214333746No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGAG41560.12098297796281667No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA41290.12019699615218238No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT39980.1163835288487346No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA38740.1127738346073031No Hit
TAGCTTATCAGACTGATGTTCTGCGGACAATTACG38680.11259917198271772No Hit
TAGCTTATCAGACTGATGTTGACATAACACCGTTG38440.11190052148437614No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA37820.11009567436366038No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA37810.1100665639262295No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC37510.10919325080330253No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT36050.10494312693839125No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA35410.1030800589428137No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA35170.10238140844447212No Hit
TAGCTTATCAGACTGATGTTCTGTATTGTGGCAAC35080.10211941450759404No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35080.10211941450759404No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT34950.10174097882099235No Hit
TAGCTTATCAGACTGATGTTCTGTATTGTGGCTTG34510.1004601195740328No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA34370.1000525734500002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)