FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536598
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1435435.658878545201093No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1007243.970830222211009No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA660232.6028168436622594No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN520902.053537848724946No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT409151.6129871583908841No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT356821.4066872243847863No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC351991.3876459730710187No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA349811.379051785107455No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN316011.2458024487916493No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT312991.2338967388604738No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT250910.9891594963017395No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA232900.9181588884009212No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT225700.8897744143928207No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA220030.8674216411114414No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA193020.7609404407004973No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA180630.7120954916782242No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA176940.6975484487490725No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172350.6794533465689084No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA162290.6397939287187012No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA157390.620476717240966No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT135630.5346925291275952No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA122840.4842706648826499No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119740.47204957190693997No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA118030.465308259330016No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA110000.4336516862348705No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA101570.4004181979170527No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA100030.3943470743097645No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA93270.36769720704660336No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT91400.3603251283806106No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA89750.35382035308708754No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC84260.3321771916559108No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT80040.3155407360567185No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA79380.3129388259393093No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA78110.30793212010732485No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA76350.30099369312756696No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT75410.2972879423542871No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT74760.2947254551174447No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA74110.2921629678806023No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC73310.28900913743525775No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA72840.2871562620486179No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTC70330.2772611190263495No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA69690.2747380546700739No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA63010.24840357045144718No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC61410.24209590956075816No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT60880.2400064968907174No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTG59530.23468440801419854No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG59140.2331469156720931No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC58210.22948058777938007No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC58040.22881039880974438No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA57760.2277065581538738No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA56330.22206908623282048No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA54160.21351432114982352No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC52690.20771915770650298No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC50900.2006624620850446No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC50630.19959804430974082No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG48980.1930932690162178No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT46260.18237024550204645No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA44900.1770087337449608No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC44800.1766145049392927No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC43870.1729481770465797No Hit
CCACTACGCCTTGGCCGTACAGCAGAAAGCTTGAC42520.16762608817006086No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT42330.16687705343929155No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA41400.16321072554657853No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA40690.16041170102633526No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG40270.15875594004252938No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39990.15765209938665883No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA39190.15449826894131433No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA37740.14878195125912738No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA36700.14468197168017952No Hit
TAATACTGCCTGGTAATGATGACGCCTTGGCCGTA36610.14432716575507826No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA35280.13908392263969302No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG34880.13750700741702074No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA34790.1371522014919195No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT34390.13557528626924722No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC33540.13222434142106868No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG33030.13021377451216154No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA32320.12741474999191832No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT32270.12721763558908428No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG32090.12650802373888176No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC29960.118110950178152No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA29440.11606096038867805No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT29020.11440519940487219No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT28950.11412923924090455No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA28300.11156675200406213No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG27190.10719081226114663No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG26520.10454947926317058No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC26500.10447063350203697No Hit
ACTGGACTTGGAGTCAGAAGGCACGCCTTGGCCGT26250.10348506148786683No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA25720.10139564881782608No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA25580.10084372848989079No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA25530.10064661408705675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)