FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568266
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT800103.1153315116113363No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA430871.6776689018972335No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT338011.3161019925506159No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC330671.287522398380853No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310281.2081303104896457No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA302551.1780321820247592No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA267901.0431162504195437No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA254300.9901622339742067No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN243030.9462804865228134No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA223200.8690688581322962No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA192220.7484427236119623No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA155460.6053111321023601No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT155440.6052332585487641No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC151370.5893859903919609No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG137090.5337842731243571No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA116880.4550930472155143No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN115490.44968083524058644No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA107620.4190375919005274No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN106250.4137032534791957No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA101820.3964542613576631No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC92670.3608271105874547No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA90960.35416892175498954No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC85160.3315855912121252No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA75520.2940505383788128No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA71890.27991648840112354No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT71110.27687941981087627No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT69620.27107784006796803No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT66700.25970830124293975No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT66410.2585791347157966No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA65850.25639867521510623No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA64590.251492641338553No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA64580.2514537045617549No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC61960.2412522690406679No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT58670.2284420694741121No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC57930.225560747991057No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCG57660.22450945501750985No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC56390.21956448436415854No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT53220.2072215261191792No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG52990.20632598025282425No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA51230.19947310753636888No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTA51200.19935629720597478No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT50740.19756520547326487No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG50730.1975262686964668No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT49620.19320428647188415No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA49150.19137425796237617No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC47090.18335328194197953No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG46090.17945960426217533No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA45570.17743489186867717No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA45400.17677296666311046No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43620.16984222039305896No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATA42590.16583173238286067No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA41750.16256104313182512No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC41680.16228848569423884No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT40810.1589009861128092No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC40570.15796650346965618No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA40350.15710989438009926No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGAGAT40140.1562922220673404No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA38830.1511915043067969No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC38060.14819337249334766No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC37410.14566248200147494No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA37290.14519524067989842No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT36820.14336521217039044No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA36770.14317052828640023No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA35030.13639552912354094No Hit
CTGACCTATGAATTGACAGTCCGCAGGTTAGCATG33900.1319956733453622No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA32300.1257657890576755No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC31730.12354639278018709No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT31730.12354639278018709No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA31240.12163849071708305No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC30540.1189129163412201No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTG30010.11684926717092389No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC28090.10937340602569982No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC27830.10836104982895073No Hit
CTGACCTATGAATTGACAGTCCGCCTTGGCTACGT27450.10688145231062514No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA27400.10668676842663494No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC26930.10485673991712696No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG26180.10193648165727383No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT25960.1010798725677169No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA25790.10041794736215018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)