FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398544
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT853142.5103102975862606No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC710672.0911013657613378No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA636681.8733904872204097No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN572461.6844272135361498No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN418671.231909900239632No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA335710.9878053660626434No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT329730.9702095956386029No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT285550.8402127499305585No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN268810.7909563624893484No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246550.7254577254259471No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC230390.6779079511696774No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT223200.6567518325494682No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA222010.6532503330838147No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA197070.5798659661313786No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT189640.5580036627449872No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC185190.5449098202053585No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA174610.5137788417628255No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA164560.4842073546789448No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA151620.44613222603561997No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC149210.43904095400854015No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA146480.4310081022932173No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA144360.4247701368586077No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA135920.3999359725811995No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC127460.3750429595732761No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCTACG121550.35765315970603884No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA120250.3538279922225518No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA107680.3168415650937578No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA107420.3160765315970604No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA103760.3053072139127815No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA101700.2992457946697174No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA98700.29041848509243956No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC97150.28585770847751274No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA96560.2841216709273148No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT96040.2825916039339199No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC94190.2771480963612653No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA91540.26935063956800326No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG91370.2688504253586242No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA89190.26243591373246894No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC86700.25510924678332836No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA85290.25096041128200786No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT85010.2501365290547952No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC84790.2494891930191282No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA83450.2455463280746108No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA79540.23404140125889203No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAGCATG73710.2168869963137155No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT72960.2146801689193961No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT72550.21347376994383477No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC70370.2070592583176796No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA70360.207029833952422No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT70350.20700040958716437No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA69140.20344006139099569No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGATGT69010.20305754464264697No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA66570.19587799951979434No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC66110.1945244787179451No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT64200.1889044249537449No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT62050.1825781864233625No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG61410.18069502704687657No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC60450.17787028798214766No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA59290.1744570616122669No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC59100.17389799867237266No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC56800.16713039466312632No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC56190.16533550838241318No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCGC54170.15939178660037945No Hit
TAGCTTATCAGACTGATGTTGACCGTTGGTTAATG54090.15915639167831871No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCTACG53440.1572438079365752No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA53430.1572143835713176No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG53360.15700841301451446No Hit
CTGACCTATGAATTGACAGCCACGCCAACCGGCAT53250.15668474499668095No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC53220.15659647190090817No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC50630.14897556129919165No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA50440.14841649835929738No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG49790.14650391461755388No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGATGG49700.14623909533023552No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC49380.14529751564199256No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC48810.1436203268223098No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT48610.14303183951715792No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT47920.14100155831438405No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG47890.14091328521861127No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA47250.13903012584212535No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC46090.1356168994722446No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG46030.13544035328069903No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA44130.12984972388175642No Hit
CAACGGAATCCCAAAAGCAGCTGTCGCCTTGGCCG42500.12505355234476884No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG41710.122729027489419No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41340.12164032597488807No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGATGCA39690.11678530570738528No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG39230.11543178490553603No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT39160.11522581434873287No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC37300.10975288241082064No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCTACG37050.10901727327938082No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA36800.108281664147941No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC35180.103514916976211No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGAGTT34840.10251448855745283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)