FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3968527
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1034672.6071890149670143No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN840312.1174355119670345No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA758091.9102553667897433No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT691531.742535706573245No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN435951.0985184175387996No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT373780.9418607962097776No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA369890.9320586706352256No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA350310.8827204653011055No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA336930.8490051850472481No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA319830.805916149745233No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN291430.734353073571126No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAA284720.7174450369116804No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA253350.6383980756588024No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTG246200.6203813152839832No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAC226110.5697579983706801No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA215710.5435518014618522No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC201690.5082238321674515No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTT201060.5066363413931667No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187030.4712831738325076No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA178320.4493354839213643No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA168640.42494356218314755No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC167780.42277651128491756No Hit
CCACTACGCCTTGGCCGTACAGCAGCCTCTTACAC164610.4147886608809767No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG153010.38555867202113026No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT131880.33231473541694434No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA130940.32994609838864647No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT128020.32258820464116783No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT117840.2969363695900267No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA105880.2667992431448747No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT104900.2643298130515428No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT100160.2523858348450193No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG97050.24454917403862944No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG95280.24008908091087702No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA93330.23517541899047179No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG89070.22444095756435573No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC87700.22098879508694286No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT84400.21267336722164165No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC83680.2108590920510305No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC79480.2002758202224654No Hit
TAGCTTATCAGACTGATGTTGACTCGCCTTGGCCG78460.1977055970640996No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC77020.1940770467228773No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG74870.1886594194773023No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA74110.18674435124165717No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA72430.18251104251023112No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA71980.18137712052859917No Hit
TCAGTGCACTACAGAACTTTGTCCGCCTTGGCCGT68900.17361605452098475No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC68410.1723813394743188No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA68110.17162539148656417No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG67450.16996230591350392No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC66430.16739208275513812No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACAC65070.1639651185439837No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC62600.15774114677813708No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC62520.15753956064806918No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT62130.15655682826398812No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA61870.15590167334126742No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA56760.1430253592831799No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA56250.14174024770399696No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC56130.14143786850889511No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA55270.1392708176106651No Hit
TTCAAGTAATCCAGCTATCGCTCGCCTTGGCCGTA53200.134054776495158No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT51880.13072860534903757No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG50150.12636930528631907No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC50110.12626851222128513No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCAC50000.12599133129244175No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA49330.12430304745312303No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA46260.11656717971176712No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT45380.11434973228102013No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC45110.11366937909204095No Hit
TACCCTGTAGATCCGAATTTGTGTCGCCTTGGCCG44550.11225827618156561No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44280.11157792299258643No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC43390.10933527729558096No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTA43100.1086045275740848No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC42990.10832734664524143No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA42780.10779818305381317No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG42280.10653826974088874No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACTG42210.10636188187707933No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC41840.10542954602551526No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT41280.10401844311503992No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTAATTAC41260.10396804658252294No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA41220.10386725351748899No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA39730.10011271184497421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)