FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078177
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT53111517.25420597970812No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2089486.7880437024901426No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC773222.511941321113113No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN567881.8448581741725705No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT488541.5871082137252017No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC416421.3528136945991085No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN415451.3496624787983276No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA397111.290081759430988No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA344021.1176095461696973No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG323221.0500370836374906No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA269930.8769151351595441No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA267830.8700929153846579No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA244470.7942038420792565No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT213610.6939496981492617No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA211760.6879396473951953No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT198610.6452195569000743No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN194270.6311203026986427No Hit
GTCCAGTTTTCCCATTCCGAAAATAAGGTTAATGC165560.537850812347698No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157970.5131933608756092No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA154510.5019529416274633No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA149640.48613188910189375No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA149240.48483241866858207No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA144750.47024586305465865No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC130310.42333498041210754No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA127870.41540821076890644No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG106800.3469586056942145No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA99140.3220737468962961No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA98600.3203194618113253No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC91210.29631174555589235No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG90290.2933229635592755No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA87510.2842916440477595No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA80980.2630777892239465No Hit
GTCCAGTTTTCCCAGCTTAGGGAGCGGAACCGGCA80240.2606737689223199No Hit
TGGAATGTAAAGAAGTATGTATCGCCTTGGCCGTA78570.2552484798632437No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA77910.2531043536482795No Hit
GTCCAGTTTCTTTGCCTTAGGGGCGGAACCGGCAT77590.2520647773016302No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA76490.24849123361002307No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC76010.24693186909004908No Hit
GTCCGACCCCTTTGAAGGCTTTCTATTCCAATTAC75900.24657451472088837No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT73990.24036954340182518No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA72630.23595134392856554No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA65050.21132637921730946No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA62550.20320468900911154No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT61380.19940373799167493No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA58770.19092469341431634No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC58520.19011252439349655No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG57510.1868313615493846No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT56710.18423242068276124No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC54920.17841729049369157No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT52900.17185496480546766No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA52600.1708803619804839No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCGCCGT52410.17026311352466086No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT52380.17016565324216248No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC52350.1700681929596641No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG51460.16717687124554564No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT49650.16129676753481037No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC47590.1546044948032553No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC47410.15401973310826506No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA44980.1461254502258967No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT43090.13998545242849908No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC41100.13352058702277356No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA40240.13072672559115345No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA40040.13007699037449763No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA38390.12471667483708701No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA38360.12461921455458864No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA38300.12442429398959189No Hit
TGAGATGAAGCACTGTAGCTCGCCTTGGCCGTACA37190.12081826353715203No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT37150.12068831649382086No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA35810.11633509054222678No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT35540.11545794799974139No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGAT35400.11500313334808232No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA35070.1139310702406002No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC34190.11107223528731454No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA34160.11097477500481617No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA32680.106166734401563No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT32080.10421752875159551No Hit
TCCCTGAGACCCTAACTTGTGAACGCCTTGGCCGT31610.10269065099245431No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA30940.10051403801665726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)