FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160909
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT34997016.195499208897736No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1423796.5888475636873185No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC467662.1641818327379823No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC388181.7963736557161825No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN387021.79100554442598No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA300321.3897855023048171No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA283321.3111149058104714No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT271011.2541481385842717No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN260201.2041228945781615No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA254591.1781615977350273No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG197980.9161885114088562No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA180470.8351577970196802No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT179690.8315482049452337No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA175670.8129449227153943No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149930.6938283842586614No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139520.6456542131112416No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT134160.620849836804789No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA120050.5555532417144823No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA107430.4971518930227974No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA100330.4642953497810412No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA97970.4533740199147674No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA96070.44458142383598753No Hit
GTCCAGTTTTCCCATTCCGAAAGATAAGGTTAATG90030.4166302236697612No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC85600.39612959175976403No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG83550.38664284335897536No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA81010.37488853070629075No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC78600.3637358167326805No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA73130.33842239539008817No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG66530.3078796932216951No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA61430.2842785142733914No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC60590.2803912612701414No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC58020.268498118153055No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT56170.2599369061816115No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC54790.25355070481912934No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA53740.24869163856506682No Hit
GTCCAGTTTTCCCAGGAATAAAATAAGGTTAATGC51000.236011789483037No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC50950.2357804053757007No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA50350.23300379608766497No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA49320.22823728347653696No Hit
GTCCAGTTTCTTTGAAGGCGGGGCGGAACCGGCAT48510.22448886093768872No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA48340.2237021549727453No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA47350.21912074964748632No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC46540.2153723271086381No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC44010.20366429127742072No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG42460.19649138394999513No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA40980.18964241437284032No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA39610.1833024898318254No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA38440.17788810172015573No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA37650.17423223282424202No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG36490.16886412153403962No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA35480.16419016256584612No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA34960.1617837678495485No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT33050.1529448949493014No Hit
GTCCAGTTTCTTTGAAGGCTTTTATTCCAATTACG32850.15201935851995618No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT32590.15081616116180738No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGAC32270.14933530287485497No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT32040.14827093598110797No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA31030.14359697701291446No Hit
GTCCAGTTTTCCCAGGAATCGGAGCGGAACCGGCA30660.14188473461862577No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT30380.14058898361754243No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA30240.13994110811700078No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA29950.13859908029445017No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC29360.1358687478278817No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGCA29350.13582247100641442No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC29180.13503576504147097No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA28920.13383256768332216No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC28810.13332352264718228No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT28720.1329070312539769No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA28590.13230543257490252No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC28250.13073202064501557No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT28010.1296213769298013No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT27260.12615061531975663No Hit
GTCCAGTTTTCCCATTCCGATTCTATTCCAATTAC27160.12568784710508402No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC26920.12457720338986972No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT25740.1191165384567328No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA23910.11064788012822382No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC23590.10916702184127142No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA23520.10884308409100059No Hit
ATCACATTGCCAGGGATTACCGCCTTGGCCGTACA22760.10532604565948868No Hit
ATCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT22280.1031047582290601No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC22030.10194783769237853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)