FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623400
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN458411.265137715957388No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN380451.049980681128222No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN377441.0416735662637302No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN365051.0074791632168683No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG328890.9076833912899487No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT301980.8334161284980957No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT276960.7643649610862726No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA265980.7340619307832422No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA208620.5757575757575757No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT189250.5222994977093337No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT183110.5053540873213004No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC141540.39062758734889885No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC140910.3888888888888889No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA139480.3849423193685489No Hit
CACATCACCCCATAAACACCACGCCTTGGCCGTAC125350.3459457967654689No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT117890.3253573991278909No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC116260.32085886184246837No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG115130.31774024396975215No Hit
GGCTCACATCACCCCATAAACACCACGCCTTGGCC111220.30694927416238893No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA109140.30120880940553074No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA107050.2954407462604184No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA104900.2895070927857813No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA92700.25583705911574767No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC91240.25180769443064527No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT88270.24361097311916985No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG88120.24319699729535796No Hit
ACTTAACTTGACCGCTCTGACCACGCCTTGGCCGT85150.23500027598388254No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA73410.20259976817353867No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA71810.19818402605287852No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA71100.1962245404868356No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAC70910.1957001711100072No Hit
ACATCACCCCATAAACACCACGCCTTGGCCGTACA70660.19501021140365402No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC64360.17762322680355466No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC63990.17660208643815203No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT63310.17472539603687146No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT62470.17240713142352487No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT59020.16288568747585142No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA58990.16280289231108905No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA58640.16183694872219462No Hit
ACACCTCTTTACAGTGACCACGCCTTGGCCGTACA58420.16122978418060385No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA57210.1578903792018546No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG56530.15601368880057406No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA56200.1551029419881879No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA55170.15226030799801293No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA54990.15176353700943865No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG52370.14453275928685763No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA52230.14414638185129988No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG51430.1419385107909698No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT49930.13779875255285093No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCAC48920.1350113153391842No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA48740.13451454435060994No Hit
TCACATCACCCCATAAACACCACGCCTTGGCCGTA48560.13401777336203566No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA48010.13249986200805874No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA47610.1313959264778937No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC47250.13040238450074518No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA46410.1280841198873986No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA45710.12615223270960976No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA43880.12110172765910472No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC43710.12063255505878458No Hit
GGAGATGAAAACCTTTTTCCAAGGACACCACGCCT43680.1205497598940222No Hit
ACTCCACCTTACTACCAGACAACCCGCCTTGGCCG43050.11881106143401227No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC43020.11872826626924987No Hit
ACTTAACTTGACCGCTCTGACCCGCCTTGGCCGTA42920.11845228238670863No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAACA41700.11508527901970525No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA41090.11340177733620356No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA38760.10697135287299221No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGAT38710.10683336093172159No Hit
CAACTTAACTTGACCGCTCTGACCACGCCTTGGCC36660.10117569133962577No Hit
GCTCACATCACCCCATAAACACCACGCCTTGGCCG36480.1006789203510515No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC36430.10054092840978088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)