FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580582
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1921067.444289698990383No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA900903.4910729440103045No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN412691.5992128907355008No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA359341.3924765808643167No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT344991.3368689698680376No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN299281.159738384596963No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN267871.0380216555800204No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA266011.0308139791721402No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC241280.9349828837060786No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC233330.9041758797046557No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA168050.6512096883571226No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150680.5838992909351457No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG144540.5601062085994555No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT136740.5298804688244745No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC135930.526741642001688No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA116860.45284358334670244No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA115780.44865848091632043No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA103850.4024285994399713No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA102270.39630594958811616No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA100540.3896020355098191No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA94220.3651114361023986No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA92590.3587950315083962No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT84940.3291505559598571No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA78500.3041949451712831No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC77910.30190863921394473No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC76270.29555348367151285No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT75310.29183339262228447No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA73010.282920674483508No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC70530.27331043927300125No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG65250.25284993850224485No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA64160.2486260851234334No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT63850.24742480572212006No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT63530.24618477537237723No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC63500.24606852252708886No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT62320.24149591061241224No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA59530.2306843960005921No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG59010.22866934668226002No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT56490.21890410767803542No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA56350.21836159440002295No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA56170.2176640773282926No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG55850.21642404697854978No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA52600.2038299887389744No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC49890.19332848171458997No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC48010.18604330340985095No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA47880.18553954108026793No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG47510.1841057559883778No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT46440.1799594045064253No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA44470.17232546766582113No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC44260.1715116977488024No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA43560.16879913135874No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA42470.16457527797992855No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC42410.1643427722893518No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT41430.16054517934326443No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC41220.1597314094262457No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA40580.15725134872676008No Hit
CTGACCTATGAATTGACAGCTGTATTCCATAATGC38970.15101244602961658No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT38510.1492299024018613No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA38410.14884239291756665No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA36070.13977467098507237No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC36040.13965841813978397No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGAGAT35540.13772087071831082No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA35330.13690710080129212No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA34840.13500830432824842No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC34570.13396202872065294No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA34120.13221823604132713No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT34100.1321407341444682No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG34010.13179197560860303No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC33690.1305519452588602No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC33560.1300481829292772No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT33370.1293119149091174No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC33330.12915691111539956No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC33180.12857564688895762No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC33020.1279556317140862No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA32700.1267156013643434No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT32340.12532056722088272No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA32190.12473930299444079No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG31960.12384803118056313No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA30920.11981793254389901No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT29860.11571033201037595No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA29300.11354027889832605No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG28880.1119127390642886No Hit
CAGTGCAATGTTAAAAGGGCATCGCCTTGGCCGTA28720.11129272388941719No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC28230.10939392741637351No Hit
TGACCTATGAATTGACAGCCACGCCTTGCGGCATG27970.10838640275720747No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG27660.10718512335589413No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT27470.10644885533573434No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATG27260.10563508541871564No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC26580.10300002092551216No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA25960.10059746212288545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)