FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875793
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT1257773.2451939512765517No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG1104072.848629944891278No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN750931.9374873735516835No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT615381.5877524934897196No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT506411.3065971273491643No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA479801.2379402099131713No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA430861.1116692764551668No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA423151.091776573207083No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA413501.0668784426825684No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC394321.0173917956918752No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN390311.0070455259091493No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT361010.9314480933321259No Hit
GGGCCGCCGGTGAAATACCACTACTCGCCTTGGCC353910.9131292615472498No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN322640.8324489981792114No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGAT311010.8024422356921539No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN256460.6616968450069444No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC245650.6338057785851824No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA229220.5914144537646876No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT217360.5608142643324863No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA207920.5364579584100595No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG163820.42267479197160424No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA160380.41379918896597423No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT157790.4071166855402236No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA150820.38913326898521156No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC150160.3874303916643639No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA149360.38536629794212435No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA147730.3811607069830613No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA140470.36242905645373735No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA139320.359461921728018No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA137370.35443069328005905No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGGC132590.34209773328967774No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA129810.3349250076048953No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA126460.3262816151430172No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA119380.30801438570119716No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC118240.3050730521470058No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC100690.2597919961153756No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCA99950.257882709422304No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG98110.2531352938611531No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA95880.24738163261041032No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG93050.24007990106798788No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC92620.23897045069228412No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC91180.23525508199225292No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG83430.21525917405805728No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGAA82650.21324668267887373No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA82130.211905021759418No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC81370.20994413272329043No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT77790.20070731331626843No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT77490.19993327817042858No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGAC76880.19835940670722094No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGCGGCATGTC74930.19332817825926205No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG66810.1723776269785306No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT66740.1721970187778346No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA63560.16399224623193243No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG63400.1635794274874845No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT60670.15653570766034203No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT60530.1561744912589501No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG59880.15449741510963047No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA57850.14925977728944761No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA57810.14915657260333562No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC57320.1478923151984639No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA56480.14572501679011238No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA56450.1456476132755284No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA56010.14451236172829662No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT55070.14208705160466517No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT52960.13664300441225835No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG50340.1298830974719238No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT48990.12639993931564455No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG48320.12467126082326893No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT46480.11992384526211797No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC45740.11801455856904638No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA45310.11690510819334264No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT45040.11620847656208677No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT44800.1155892484454149No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA44680.11527963438707899No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC43450.11210609028913567No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCGAACCGGCA43420.11202868677455168No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC42680.11011940008148009No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG42650.1100419965668961No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA42300.10913895556341631No Hit
TAATACTGCCTGGTAATGATGACGCCTTGGCCGTA41960.1082617157314645No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG41900.10810690870229653No Hit
ATCACATTGCCAGGGATTTCCATCGCCTTGGCCGT39790.10266286150988971No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC39600.10217263925085783No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGTA39560.10206943456474585No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT39260.10129539941890602No Hit
TAGCAGCACATCATGGTTTACCGCCTTGGCCGTAC38980.10057296661612218No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTAC38840.10021175021473025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)