FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417755
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1750455.121636863964795No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1151363.368761072692455No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA1002542.933329042017348No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA765292.239159916377856No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA627551.8361468273764503No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN420541.2304568349691538No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA389001.1381740352950986No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA353611.0346265311586116No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT321680.9412026315519983No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT303390.887687970612288No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA299860.8773595532740059No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297300.8698692562808042No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA287220.8403762118700727No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA246190.7203266471704379No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT229820.6724297089756287No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC224280.6562202381387782No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT222820.6519484281348429No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC220270.6444873901142709No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA211040.6174813583770633No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA185620.543105049952381No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC177870.5204293461643681No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT175410.5132316388974634No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC170570.49907029614469145No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN164130.48022751777116846No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA157380.460477711246125No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157150.45980475487564204No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA154960.453397039869739No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA142470.41685258305525114No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGGC133330.3901098820717108No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA132440.38750583350766804No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT114940.33630263140570343No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA109380.32003464262359355No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT89110.2607267051032037No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT88480.258883389827533No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA82610.2417083728939026No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC82390.24106467549604932No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA80320.23500806816170264No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC78140.22862961212842936No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA74110.21683824615866265No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA74000.21651639745973603No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT68340.19995581895132916No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC67500.19749806525043487No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA65290.19103183229927248No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA64060.18743297866582012No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC63650.18623336078800265No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGCTTA58930.17242312570678706No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC58350.1707261052942648No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG56610.16563504405669804No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA56310.16475727487780722No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA56120.16420135439784303No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC54350.1590225162423872No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC53160.1555406984994536No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT51810.15159073719444488No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG49930.14609005034006242No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT49160.14383710944757597No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA48170.14094047115723626No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT47360.13857049437423105No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACC46470.13596644581018827No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT46170.13508867663129745No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC45590.1333916562187752No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA45530.13321610238299703No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC43760.1280372642275412No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43030.12590135922557352No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT42840.12534543874560933No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA42520.12440915162145912No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA41080.12019585956278317No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT40670.11899624168496571No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40550.11864513401340938No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC40230.11770884688925919No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA39920.11680181873773866No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG39520.1156314598325509No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC39210.11472443168103039No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC37930.11097928318442955No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA37900.11089150626654046No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC36750.10752672441412564No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG36560.10697080393416146No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC36020.10539081941215798No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA35730.10454230920589686No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA35720.10451305023326715No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT34770.10173344783344623No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG34250.10021198125670214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)