FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897782
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT845892.9190946730982525No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC672872.3220173222140246No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT521631.8001009047609515No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA401981.387198899020009No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN371311.2813593293077257No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA339131.170308877617433No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT336511.1612674797483042No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA199280.6876983844885503No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN199200.6874223112711723No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA196050.6765519283369142No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA182880.6311033749260642No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT173430.5984922261232902No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170860.5896233740150225No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG165530.5712299959072145No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT164340.5671234067987171No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA155170.5354785142567661No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131410.4534847686955058No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA124470.4295354170879659No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA122480.42266809580568865No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA119610.41276396913225355No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC114100.39374942628534515No Hit
GCCGCCGGTGAAATACCACGCAGATAAGGTTAATG105530.3641750828737289No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA102540.35385684637422693No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT94620.3265255978538068No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT84510.29163684500766446No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC83140.2869090911600666No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC63120.21782176851122687No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA59850.2065372757509019No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA58590.2021891225771987No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA58250.20101581140334226No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA57480.19835860668607924No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA55400.19118070303425172No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA54150.18686705901272077No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA51230.17679038657842447No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA49890.1721661601873433No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC49140.1695779737744247No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC47160.16274516164431968No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA46070.1589836640575447No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA44980.15522216647076972No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA44610.15394532784039655No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG44100.15218536107961192No Hit
TAGCACCATCTGATTAGCCAATGCGGAACCGGCAT43450.14994226618841583No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC40270.1389683557976411No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC40150.13855424597157412No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA39800.13734642564554544No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCA38860.13410256534135417No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA37740.13023754029806245No Hit
GCCGCCGGTGAAATACCACTACTCCGCCTTGGCCG37600.129754412167651No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG34640.11953970312466569No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG34250.11819384618994805No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC33850.11681348010305814No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA33760.1165028977335079No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA33440.11539860486399597No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT31940.11022223203815884No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT31770.10963557645123063No Hit
GAGCCCAGCGCCGAATCCCCCGCCTTGGCCGTACA31580.10897990255995793No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA30740.10608113377748912No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA29050.10024908706037929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)