@HD	VN:1.4	SO:coordinate
@SQ	SN:1	LN:249250621	AS:NCBI37	M5:1b22b98cdeb4a9304cb5d48026a85128	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:2	LN:243199373	AS:NCBI37	M5:a0d9851da00400dec1098a9255ac712e	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:3	LN:198022430	AS:NCBI37	M5:fdfd811849cc2fadebc929bb925902e5	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:4	LN:191154276	AS:NCBI37	M5:23dccd106897542ad87d2765d28a19a1	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:5	LN:180915260	AS:NCBI37	M5:0740173db9ffd264d728f32784845cd7	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:6	LN:171115067	AS:NCBI37	M5:1d3a93a248d92a729ee764823acbbc6b	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:7	LN:159138663	AS:NCBI37	M5:618366e953d6aaad97dbe4777c29375e	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:8	LN:146364022	AS:NCBI37	M5:96f514a9929e410c6651697bded59aec	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:9	LN:141213431	AS:NCBI37	M5:3e273117f15e0a400f01055d9f393768	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:10	LN:135534747	AS:NCBI37	M5:988c28e000e84c26d552359af1ea2e1d	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:11	LN:135006516	AS:NCBI37	M5:98c59049a2df285c76ffb1c6db8f8b96	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:12	LN:133851895	AS:NCBI37	M5:51851ac0e1a115847ad36449b0015864	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:13	LN:115169878	AS:NCBI37	M5:283f8d7892baa81b510a015719ca7b0b	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:14	LN:107349540	AS:NCBI37	M5:98f3cae32b2a2e9524bc19813927542e	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:15	LN:102531392	AS:NCBI37	M5:e5645a794a8238215b2cd77acb95a078	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:16	LN:90354753	AS:NCBI37	M5:fc9b1a7b42b97a864f56b348b06095e6	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:17	LN:81195210	AS:NCBI37	M5:351f64d4f4f9ddd45b35336ad97aa6de	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:18	LN:78077248	AS:NCBI37	M5:b15d4b2d29dde9d3e4f93d1d0f2cbc9c	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:19	LN:59128983	AS:NCBI37	M5:1aacd71f30db8e561810913e0b72636d	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:20	LN:63025520	AS:NCBI37	M5:0dec9660ec1efaaf33281c0d5ea2560f	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:21	LN:48129895	AS:NCBI37	M5:2979a6085bfe28e3ad6f552f361ed74d	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:22	LN:51304566	AS:NCBI37	M5:a718acaa6135fdca8357d5bfe94211dd	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:X	LN:155270560	AS:NCBI37	M5:7e0e2e580297b7764e31dbc80c2540dd	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:Y	LN:59373566	AS:NCBI37	M5:1fa3474750af0948bdf97d5a0ee52e51	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:MT	LN:16569	AS:NCBI37	M5:c68f52674c9fb33aef52dcf399755519	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000207.1	LN:4262	AS:NCBI37	M5:f3814841f1939d3ca19072d9e89f3fd7	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000226.1	LN:15008	AS:NCBI37	M5:1c1b2cd1fccbc0a99b6a447fa24d1504	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000229.1	LN:19913	AS:NCBI37	M5:d0f40ec87de311d8e715b52e4c7062e1	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000231.1	LN:27386	AS:NCBI37	M5:ba8882ce3a1efa2080e5d29b956568a4	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000210.1	LN:27682	AS:NCBI37	M5:851106a74238044126131ce2a8e5847c	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000239.1	LN:33824	AS:NCBI37	M5:99795f15702caec4fa1c4e15f8a29c07	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000235.1	LN:34474	AS:NCBI37	M5:118a25ca210cfbcdfb6c2ebb249f9680	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000201.1	LN:36148	AS:NCBI37	M5:dfb7e7ec60ffdcb85cb359ea28454ee9	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000247.1	LN:36422	AS:NCBI37	M5:7de00226bb7df1c57276ca6baabafd15	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000245.1	LN:36651	AS:NCBI37	M5:89bc61960f37d94abf0df2d481ada0ec	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000197.1	LN:37175	AS:NCBI37	M5:6f5efdd36643a9b8c8ccad6f2f1edc7b	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000203.1	LN:37498	AS:NCBI37	M5:96358c325fe0e70bee73436e8bb14dbd	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000246.1	LN:38154	AS:NCBI37	M5:e4afcd31912af9d9c2546acf1cb23af2	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000249.1	LN:38502	AS:NCBI37	M5:1d78abec37c15fe29a275eb08d5af236	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000196.1	LN:38914	AS:NCBI37	M5:d92206d1bb4c3b4019c43c0875c06dc0	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000248.1	LN:39786	AS:NCBI37	M5:5a8e43bec9be36c7b49c84d585107776	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000244.1	LN:39929	AS:NCBI37	M5:0996b4475f353ca98bacb756ac479140	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000238.1	LN:39939	AS:NCBI37	M5:131b1efc3270cc838686b54e7c34b17b	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000202.1	LN:40103	AS:NCBI37	M5:06cbf126247d89664a4faebad130fe9c	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000234.1	LN:40531	AS:NCBI37	M5:93f998536b61a56fd0ff47322a911d4b	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000232.1	LN:40652	AS:NCBI37	M5:3e06b6741061ad93a8587531307057d8	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000206.1	LN:41001	AS:NCBI37	M5:43f69e423533e948bfae5ce1d45bd3f1	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000240.1	LN:41933	AS:NCBI37	M5:445a86173da9f237d7bcf41c6cb8cc62	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000236.1	LN:41934	AS:NCBI37	M5:fdcd739913efa1fdc64b6c0cd7016779	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000241.1	LN:42152	AS:NCBI37	M5:ef4258cdc5a45c206cea8fc3e1d858cf	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000243.1	LN:43341	AS:NCBI37	M5:cc34279a7e353136741c9fce79bc4396	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000242.1	LN:43523	AS:NCBI37	M5:2f8694fc47576bc81b5fe9e7de0ba49e	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000230.1	LN:43691	AS:NCBI37	M5:b4eb71ee878d3706246b7c1dbef69299	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000237.1	LN:45867	AS:NCBI37	M5:e0c82e7751df73f4f6d0ed30cdc853c0	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000233.1	LN:45941	AS:NCBI37	M5:7fed60298a8d62ff808b74b6ce820001	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000204.1	LN:81310	AS:NCBI37	M5:efc49c871536fa8d79cb0a06fa739722	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000198.1	LN:90085	AS:NCBI37	M5:868e7784040da90d900d2d1b667a1383	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000208.1	LN:92689	AS:NCBI37	M5:aa81be49bf3fe63a79bdc6a6f279abf6	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000191.1	LN:106433	AS:NCBI37	M5:d75b436f50a8214ee9c2a51d30b2c2cc	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000227.1	LN:128374	AS:NCBI37	M5:a4aead23f8053f2655e468bcc6ecdceb	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000228.1	LN:129120	AS:NCBI37	M5:c5a17c97e2c1a0b6a9cc5a6b064b714f	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000214.1	LN:137718	AS:NCBI37	M5:46c2032c37f2ed899eb41c0473319a69	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000221.1	LN:155397	AS:NCBI37	M5:3238fb74ea87ae857f9c7508d315babb	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000209.1	LN:159169	AS:NCBI37	M5:f40598e2a5a6b26e84a3775e0d1e2c81	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000218.1	LN:161147	AS:NCBI37	M5:1d708b54644c26c7e01c2dad5426d38c	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000220.1	LN:161802	AS:NCBI37	M5:fc35de963c57bf7648429e6454f1c9db	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000213.1	LN:164239	AS:NCBI37	M5:9d424fdcc98866650b58f004080a992a	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000211.1	LN:166566	AS:NCBI37	M5:7daaa45c66b288847b9b32b964e623d3	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000199.1	LN:169874	AS:NCBI37	M5:569af3b73522fab4b40995ae4944e78e	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000217.1	LN:172149	AS:NCBI37	M5:6d243e18dea1945fb7f2517615b8f52e	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000216.1	LN:172294	AS:NCBI37	M5:642a232d91c486ac339263820aef7fe0	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000215.1	LN:172545	AS:NCBI37	M5:5eb3b418480ae67a997957c909375a73	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000205.1	LN:174588	AS:NCBI37	M5:d22441398d99caf673e9afb9a1908ec5	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000219.1	LN:179198	AS:NCBI37	M5:f977edd13bac459cb2ed4a5457dba1b3	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000224.1	LN:179693	AS:NCBI37	M5:d5b2fc04f6b41b212a4198a07f450e20	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000223.1	LN:180455	AS:NCBI37	M5:399dfa03bf32022ab52a846f7ca35b30	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000195.1	LN:182896	AS:NCBI37	M5:5d9ec007868d517e73543b005ba48535	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000212.1	LN:186858	AS:NCBI37	M5:563531689f3dbd691331fd6c5730a88b	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000222.1	LN:186861	AS:NCBI37	M5:6fe9abac455169f50470f5a6b01d0f59	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000200.1	LN:187035	AS:NCBI37	M5:75e4c8d17cd4addf3917d1703cacaf25	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000193.1	LN:189789	AS:NCBI37	M5:dbb6e8ece0b5de29da56601613007c2a	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000194.1	LN:191469	AS:NCBI37	M5:6ac8f815bf8e845bb3031b73f812c012	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000225.1	LN:211173	AS:NCBI37	M5:63945c3e6962f28ffd469719a747e73c	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:GL000192.1	LN:547496	AS:NCBI37	M5:325ba9e808f669dfeee210fdd7b470ac	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:NC_007605	LN:171823	AS:NCBI37	M5:6743bd63b3ff2b5b8985d8933c53290a	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@SQ	SN:hs37d5	LN:35477943	AS:NCBI37	M5:5b6a4b3a81a2d3c134b7d14bf6ad39f1	SP:Human	UR:/nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa
@RG	ID:24311_1#2	PL:ILLUMINA	CN:SC	DT:2017-11-10T00:00:00+0000	LB:19888979	SM:EGAN00001598920	PG:SCS	DS:EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth.	PU:171110_HS36_24311_B_CBN70ANXX_1#2
@RG	ID:24311_2#2	PL:ILLUMINA	CN:SC	DT:2017-11-10T00:00:00+0000	LB:19888979	SM:EGAN00001598920	PG:SCS	DS:EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth.	PU:171110_HS36_24311_B_CBN70ANXX_2#2
@RG	ID:24311_3#2	PL:ILLUMINA	CN:SC	DT:2017-11-10T00:00:00+0000	LB:19888979	SM:EGAN00001598920	PG:SCS	DS:EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth.	PU:171110_HS36_24311_B_CBN70ANXX_3#2
@RG	ID:24311_4#2	PL:ILLUMINA	CN:SC	DT:2017-11-10T00:00:00+0000	LB:19888979	SM:EGAN00001598920	PG:SCS	DS:EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth.	PU:171110_HS36_24311_B_CBN70ANXX_4#2
@PG	ID:SCS_0	PN:RTA	DS:Controlling software on instrument	VN:1.18.66.3
@PG	ID:basecalling_0	PN:RTA	PP:SCS_0	DS:Basecalling Package	VN:1.18.66.3
@PG	ID:bambi_0	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi i2b --intensity-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities --basecalls-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BaseCalls --lane=1 --platform-unit=171110_HS36_24311_B_CBN70ANXX_1 --read-group-id=24311_1 --study-name="EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth." --sample-alias=EGAN00001598919,EGAN00001598920,EGAN00001598921,EGAN00001598923,EGAN00001598924,EGAN00001598935,EGAN00001598936,EGAN00001588950,EGAN00001588951,EGAN00001588952,EGAN00001588953,EGAN00001588954,EGAN00001588955,EGAN00001588956,EGAN00001588957,EGAN00001588958,EGAN00001588960,EGAN00001588961,EGAN00001588962,EGAN00001588963,EGAN00001588964,EGAN00001588966,EGAN00001588967,EGAN00001588969 --output-file=- --compression-level=0	PP:basecalling_0	DS:Convert Illumina BCL to BAM or SAM file	VN:0.9.12
@PG	ID:bamadapterfind_0	PN:bamadapterfind	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterfind level=0	PP:bambi_0	VN:2.0.76
@PG	ID:bambi.1_0	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi decode --metrics-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/24311_1.bam.tag_decode.metrics --barcode-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/metadata_cache_24311/lane_1.taglist --compression-level 0 -	PP:bamadapterfind_0	VN:0.9.12
@PG	ID:bamcollate2_0	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=2 level=0	PP:bambi.1_0	VN:2.0.76
@PG	ID:bwa_0	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa sampe /lustre/scratch117/core/sciops_repository/references/PhiX/Sanger-SNPs/all/bwa0_6/phix_unsnipped_short_no_N.fa /tmp/Z3J9MrD8_Y/alnphix_bwa_aln_1_out /tmp/DxlfUDtmLO/alnphix_bwa_aln_2_out /tmp/_QNU8K6Wu5/alnphix_simple_cat1_out /tmp/XrVClkWpCC/alnphix_simple_cat2_out	PP:bamcollate2_0	VN:0.7.16a-r1181
@PG	ID:scramble_0	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -t 2 -I sam -O bam 	PP:bwa_0	VN:1.14.9
@PG	ID:bam12auxmerge_0	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=1 zztoname=0 clipreinsert=1 /tmp/b4CZ_A1qO1/simple_cat_out	PP:scramble_0	VN:2.0.76
@PG	ID:scramble.1_0	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I bam -O cram -x -3 	PP:bam12auxmerge_0	VN:1.14.9
@PG	ID:scramble.2_0	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I cram -O bam -0 	PP:scramble.1_0	VN:1.14.9
@PG	ID:spf_0	PN:spatial_filter	CL:/software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -c  -F /dev/stdout -t /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/qc/tileviz/24311_1 --region_size 200 --region_mismatch_threshold 0.0160 --region_insertion_threshold 0.0160 --region_deletion_threshold 0.0160 /dev/stdin ; /software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -a  -f  -u  -l /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/24311_1.bam.filter.stats -F /tmp/YOoK7_ku_h/tee_post_filter_creation:__APPLY_FILTER_OUT___out /dev/stdin	PP:scramble.2_0	DS:A program to apply a spatial filter	VN:v10.28-dirty
@PG	ID:samtools_0	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools split -f /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/lane1/%!.bam -	PP:spf_0	VN:1.6
@PG	ID:bamcollate2'_0	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=1 level=0	PP:samtools_0	VN:2.0.76
@PG	ID:bamreset_0	PN:bamreset	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamreset resetaux=0 level=0 verbose=0	PP:bamcollate2'_0	VN:2.0.76
@PG	ID:bamadapterclip_0	PN:bamadapterclip	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterclip verbose=0 level=0	PP:bamreset_0	VN:2.0.76
@PG	ID:bwa'_0	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa mem -t 16 -p -Y -K 100000000 /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/bwa0_6/hs37d5.fa /tmp/Vq6gXngeoA/alntgt_bamtofastq_out	PP:bamadapterclip_0	VN:0.7.16a-r1181
@PG	ID:scramble'_0	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -I sam -O bam 	PP:bwa'_0	VN:1.14.9
@PG	ID:samtools'_0	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools reheader /tmp/nQBPIBDLYa/postalntgt_alterSQ_headerSQfix_out /tmp/ZcO3FBfIZu/postalntgt_mbuffer_headerSQfix_out	PP:scramble'_0	VN:1.6
@PG	ID:bam12split_0	PN:bam12split	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12split verbose=0 level=0	PP:samtools'_0	VN:2.0.76
@PG	ID:bamsormadup_0	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=queryname level=0	PP:bam12split_0	VN:2.0.76
@PG	ID:bam12auxmerge'_0	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/tLHftNsr52/amp_bamadapterclip_pre_auxmerge_out	PP:bamsormadup_0	VN:2.0.76
@PG	ID:AlignmentFilter_0	PN:AlignmentFilter	CL:uk.ac.sanger.npg.picard.AlignmentFilter INPUT_ALIGNMENT=[./initial_phix_aln_24311_1#2.bam, /tmp/NXSLuMeKjs/postalntgt_bam12auxmerge_out] OUTPUT_ALIGNMENT=[/tmp/o4DofsW4MT/alignment_filter:__PHIX_BAM_OUT___out, /dev/stdout] METRICS_FILE=24311_1#2.bam_alignment_filter_metrics.json VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=SILENT COMPRESSION_LEVEL=0 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false   	PP:bam12auxmerge'_0	DS:Give a list of SAM/BAM files with the same set of records and in the same order but aligned with different references, split reads into different files according to alignments. You have option to put unaligned reads into one of output files or a separate file	VN:V1.19
@PG	ID:bamsormadup'_0	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane1/bsfopt_24311_1#2.tmp	PP:AlignmentFilter_0	VN:2.0.76
@PG	ID:bamstreamingmarkduplicates_0	PN:bamstreamingmarkduplicates	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane1/bmdfopt_24311_1#2.tmp M=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane1/24311_1#2.markdups_metrics.txt	PP:bamsormadup'_0	VN:2.0.76
@PG	ID:scramble.3_0	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -t 7 -7 -I bam -O cram -r /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa 	PP:bamstreamingmarkduplicates_0	VN:1.14.9
@PG	ID:SCS_1	PN:RTA	PP:scramble.3_0	DS:Controlling software on instrument	VN:1.18.66.3
@PG	ID:basecalling_1	PN:RTA	PP:SCS_1	DS:Basecalling Package	VN:1.18.66.3
@PG	ID:bambi_1	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi i2b --intensity-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities --basecalls-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BaseCalls --lane=2 --platform-unit=171110_HS36_24311_B_CBN70ANXX_2 --read-group-id=24311_2 --study-name="EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth." --sample-alias=EGAN00001598919,EGAN00001598920,EGAN00001598921,EGAN00001598923,EGAN00001598924,EGAN00001598935,EGAN00001598936,EGAN00001588950,EGAN00001588951,EGAN00001588952,EGAN00001588953,EGAN00001588954,EGAN00001588955,EGAN00001588956,EGAN00001588957,EGAN00001588958,EGAN00001588960,EGAN00001588961,EGAN00001588962,EGAN00001588963,EGAN00001588964,EGAN00001588966,EGAN00001588967,EGAN00001588969 --output-file=- --compression-level=0	PP:basecalling_1	DS:Convert Illumina BCL to BAM or SAM file	VN:0.9.12
@PG	ID:bamadapterfind_1	PN:bamadapterfind	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterfind level=0	PP:bambi_1	VN:2.0.76
@PG	ID:bambi.1_1	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi decode --metrics-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/24311_2.bam.tag_decode.metrics --barcode-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/metadata_cache_24311/lane_2.taglist --compression-level 0 -	PP:bamadapterfind_1	VN:0.9.12
@PG	ID:bamcollate2_1	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=2 level=0	PP:bambi.1_1	VN:2.0.76
@PG	ID:bwa_1	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa sampe /lustre/scratch117/core/sciops_repository/references/PhiX/Sanger-SNPs/all/bwa0_6/phix_unsnipped_short_no_N.fa /tmp/VtPbhnp1Jx/alnphix_bwa_aln_1_out /tmp/OivrkQGaMG/alnphix_bwa_aln_2_out /tmp/Hudwk4939u/alnphix_simple_cat1_out /tmp/_oVPxCpozM/alnphix_simple_cat2_out	PP:bamcollate2_1	VN:0.7.16a-r1181
@PG	ID:scramble_1	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -t 2 -I sam -O bam 	PP:bwa_1	VN:1.14.9
@PG	ID:bam12auxmerge_1	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=1 zztoname=0 clipreinsert=1 /tmp/qXBeLi_fFP/simple_cat_out	PP:scramble_1	VN:2.0.76
@PG	ID:scramble.1_1	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I bam -O cram -x -3 	PP:bam12auxmerge_1	VN:1.14.9
@PG	ID:scramble.2_1	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I cram -O bam -0 	PP:scramble.1_1	VN:1.14.9
@PG	ID:spf_1	PN:spatial_filter	CL:/software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -c  -F /dev/stdout -t /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/qc/tileviz/24311_2 --region_size 200 --region_mismatch_threshold 0.0160 --region_insertion_threshold 0.0160 --region_deletion_threshold 0.0160 /dev/stdin ; /software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -a  -f  -u  -l /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/24311_2.bam.filter.stats -F /tmp/BlqN9VP5KX/tee_post_filter_creation:__APPLY_FILTER_OUT___out /dev/stdin	PP:scramble.2_1	DS:A program to apply a spatial filter	VN:v10.28-dirty
@PG	ID:samtools_1	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools split -f /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/lane2/%!.bam -	PP:spf_1	VN:1.6
@PG	ID:bamcollate2'_1	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=1 level=0	PP:samtools_1	VN:2.0.76
@PG	ID:bamreset_1	PN:bamreset	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamreset resetaux=0 level=0 verbose=0	PP:bamcollate2'_1	VN:2.0.76
@PG	ID:bamadapterclip_1	PN:bamadapterclip	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterclip verbose=0 level=0	PP:bamreset_1	VN:2.0.76
@PG	ID:bwa'_1	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa mem -t 16 -p -Y -K 100000000 /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/bwa0_6/hs37d5.fa /tmp/iC4Oi3fIp2/alntgt_bamtofastq_out	PP:bamadapterclip_1	VN:0.7.16a-r1181
@PG	ID:scramble'_1	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -I sam -O bam 	PP:bwa'_1	VN:1.14.9
@PG	ID:samtools'_1	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools reheader /tmp/Z_ETKaEwBv/postalntgt_alterSQ_headerSQfix_out /tmp/vzsqwvkrru/postalntgt_mbuffer_headerSQfix_out	PP:scramble'_1	VN:1.6
@PG	ID:bam12split_1	PN:bam12split	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12split verbose=0 level=0	PP:samtools'_1	VN:2.0.76
@PG	ID:bamsormadup_1	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=queryname level=0	PP:bam12split_1	VN:2.0.76
@PG	ID:bam12auxmerge'_1	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/ANn1db0WO0/amp_bamadapterclip_pre_auxmerge_out	PP:bamsormadup_1	VN:2.0.76
@PG	ID:AlignmentFilter_1	PN:AlignmentFilter	CL:uk.ac.sanger.npg.picard.AlignmentFilter INPUT_ALIGNMENT=[./initial_phix_aln_24311_2#2.bam, /tmp/wK3zcyaPI6/postalntgt_bam12auxmerge_out] OUTPUT_ALIGNMENT=[/tmp/L_ixR7ATfg/alignment_filter:__PHIX_BAM_OUT___out, /dev/stdout] METRICS_FILE=24311_2#2.bam_alignment_filter_metrics.json VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=SILENT COMPRESSION_LEVEL=0 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false   	PP:bam12auxmerge'_1	DS:Give a list of SAM/BAM files with the same set of records and in the same order but aligned with different references, split reads into different files according to alignments. You have option to put unaligned reads into one of output files or a separate file	VN:V1.19
@PG	ID:bamsormadup'_1	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane2/bsfopt_24311_2#2.tmp	PP:AlignmentFilter_1	VN:2.0.76
@PG	ID:bamstreamingmarkduplicates_1	PN:bamstreamingmarkduplicates	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane2/bmdfopt_24311_2#2.tmp M=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane2/24311_2#2.markdups_metrics.txt	PP:bamsormadup'_1	VN:2.0.76
@PG	ID:scramble.3_1	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -t 7 -7 -I bam -O cram -r /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa 	PP:bamstreamingmarkduplicates_1	VN:1.14.9
@PG	ID:SCS_2	PN:RTA	PP:scramble.3_1	DS:Controlling software on instrument	VN:1.18.66.3
@PG	ID:basecalling_2	PN:RTA	PP:SCS_2	DS:Basecalling Package	VN:1.18.66.3
@PG	ID:bambi_2	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi i2b --intensity-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities --basecalls-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BaseCalls --lane=3 --platform-unit=171110_HS36_24311_B_CBN70ANXX_3 --read-group-id=24311_3 --study-name="EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth." --sample-alias=EGAN00001598919,EGAN00001598920,EGAN00001598921,EGAN00001598923,EGAN00001598924,EGAN00001598935,EGAN00001598936,EGAN00001588950,EGAN00001588951,EGAN00001588952,EGAN00001588953,EGAN00001588954,EGAN00001588955,EGAN00001588956,EGAN00001588957,EGAN00001588958,EGAN00001588960,EGAN00001588961,EGAN00001588962,EGAN00001588963,EGAN00001588964,EGAN00001588966,EGAN00001588967,EGAN00001588969 --output-file=- --compression-level=0	PP:basecalling_2	DS:Convert Illumina BCL to BAM or SAM file	VN:0.9.12
@PG	ID:bamadapterfind_2	PN:bamadapterfind	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterfind level=0	PP:bambi_2	VN:2.0.76
@PG	ID:bambi.1_2	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi decode --metrics-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/24311_3.bam.tag_decode.metrics --barcode-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/metadata_cache_24311/lane_3.taglist --compression-level 0 -	PP:bamadapterfind_2	VN:0.9.12
@PG	ID:bamcollate2_2	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=2 level=0	PP:bambi.1_2	VN:2.0.76
@PG	ID:bwa_2	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa sampe /lustre/scratch117/core/sciops_repository/references/PhiX/Sanger-SNPs/all/bwa0_6/phix_unsnipped_short_no_N.fa /tmp/BeCI0YuTGc/alnphix_bwa_aln_1_out /tmp/IPRGow2gKw/alnphix_bwa_aln_2_out /tmp/looC4H20Ia/alnphix_simple_cat1_out /tmp/YZ0ViEwNwy/alnphix_simple_cat2_out	PP:bamcollate2_2	VN:0.7.16a-r1181
@PG	ID:scramble_2	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -t 2 -I sam -O bam 	PP:bwa_2	VN:1.14.9
@PG	ID:bam12auxmerge_2	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=1 zztoname=0 clipreinsert=1 /tmp/4ftpTpz3o7/simple_cat_out	PP:scramble_2	VN:2.0.76
@PG	ID:scramble.1_2	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I bam -O cram -x -3 	PP:bam12auxmerge_2	VN:1.14.9
@PG	ID:scramble.2_2	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I cram -O bam -0 	PP:scramble.1_2	VN:1.14.9
@PG	ID:spf_2	PN:spatial_filter	CL:/software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -c  -F /dev/stdout -t /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/qc/tileviz/24311_3 --region_size 200 --region_mismatch_threshold 0.0160 --region_insertion_threshold 0.0160 --region_deletion_threshold 0.0160 /dev/stdin ; /software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -a  -f  -u  -l /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/24311_3.bam.filter.stats -F /tmp/JtwPuWpEjB/tee_post_filter_creation:__APPLY_FILTER_OUT___out /dev/stdin	PP:scramble.2_2	DS:A program to apply a spatial filter	VN:v10.28-dirty
@PG	ID:samtools_2	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools split -f /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/lane3/%!.bam -	PP:spf_2	VN:1.6
@PG	ID:bamcollate2'_2	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=1 level=0	PP:samtools_2	VN:2.0.76
@PG	ID:bamreset_2	PN:bamreset	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamreset resetaux=0 level=0 verbose=0	PP:bamcollate2'_2	VN:2.0.76
@PG	ID:bamadapterclip_2	PN:bamadapterclip	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterclip verbose=0 level=0	PP:bamreset_2	VN:2.0.76
@PG	ID:bwa'_2	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa mem -t 16 -p -Y -K 100000000 /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/bwa0_6/hs37d5.fa /tmp/6TqkvfV3uK/alntgt_bamtofastq_out	PP:bamadapterclip_2	VN:0.7.16a-r1181
@PG	ID:scramble'_2	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -I sam -O bam 	PP:bwa'_2	VN:1.14.9
@PG	ID:samtools'_2	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools reheader /tmp/DaRhZfNW05/postalntgt_alterSQ_headerSQfix_out /tmp/2eZHP1quaI/postalntgt_mbuffer_headerSQfix_out	PP:scramble'_2	VN:1.6
@PG	ID:bam12split_2	PN:bam12split	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12split verbose=0 level=0	PP:samtools'_2	VN:2.0.76
@PG	ID:bamsormadup_2	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=queryname level=0	PP:bam12split_2	VN:2.0.76
@PG	ID:bam12auxmerge'_2	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/_FrZb0ZkAj/amp_bamadapterclip_pre_auxmerge_out	PP:bamsormadup_2	VN:2.0.76
@PG	ID:AlignmentFilter_2	PN:AlignmentFilter	CL:uk.ac.sanger.npg.picard.AlignmentFilter INPUT_ALIGNMENT=[./initial_phix_aln_24311_3#2.bam, /tmp/_s7UjjzBw2/postalntgt_bam12auxmerge_out] OUTPUT_ALIGNMENT=[/tmp/u0KqQ6U64V/alignment_filter:__PHIX_BAM_OUT___out, /dev/stdout] METRICS_FILE=24311_3#2.bam_alignment_filter_metrics.json VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=SILENT COMPRESSION_LEVEL=0 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false   	PP:bam12auxmerge'_2	DS:Give a list of SAM/BAM files with the same set of records and in the same order but aligned with different references, split reads into different files according to alignments. You have option to put unaligned reads into one of output files or a separate file	VN:V1.19
@PG	ID:bamsormadup'_2	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane3/bsfopt_24311_3#2.tmp	PP:AlignmentFilter_2	VN:2.0.76
@PG	ID:bamstreamingmarkduplicates_2	PN:bamstreamingmarkduplicates	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane3/bmdfopt_24311_3#2.tmp M=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane3/24311_3#2.markdups_metrics.txt	PP:bamsormadup'_2	VN:2.0.76
@PG	ID:scramble.3_2	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -t 7 -7 -I bam -O cram -r /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa 	PP:bamstreamingmarkduplicates_2	VN:1.14.9
@PG	ID:SCS_3	PN:RTA	PP:scramble.3_2	DS:Controlling software on instrument	VN:1.18.66.3
@PG	ID:basecalling_3	PN:RTA	PP:SCS_3	DS:Basecalling Package	VN:1.18.66.3
@PG	ID:bambi_3	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi i2b --intensity-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities --basecalls-dir=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BaseCalls --lane=4 --platform-unit=171110_HS36_24311_B_CBN70ANXX_4 --read-group-id=24311_4 --study-name="EGAS00001001713: The Prenatal Assessment of Genomes and Exomes (PAGE) study is a multicentre prospective trial, performing exome sequence analysis on samples from 1000 families with structural anomalies in prenatal ultrasound screening but normal aneuploidy results.  The data will enable discovery of novel genetic disorders and increase the diagnostic yield.  Where appropriate, results will be reported back to the families at the end of the pregnancy, after thorough clinical review.  Ultimately, the translation of the acquired know-how into cost-effective prenatal diagnostic sequencing will improve genetics-derived prognoses and allow more informed parental counselling as well as management of pregnancy and childbirth." --sample-alias=EGAN00001598919,EGAN00001598920,EGAN00001598921,EGAN00001598923,EGAN00001598924,EGAN00001598935,EGAN00001598936,EGAN00001588950,EGAN00001588951,EGAN00001588952,EGAN00001588953,EGAN00001588954,EGAN00001588955,EGAN00001588956,EGAN00001588957,EGAN00001588958,EGAN00001588960,EGAN00001588961,EGAN00001588962,EGAN00001588963,EGAN00001588964,EGAN00001588966,EGAN00001588967,EGAN00001588969 --output-file=- --compression-level=0	PP:basecalling_3	DS:Convert Illumina BCL to BAM or SAM file	VN:0.9.12
@PG	ID:bamadapterfind_3	PN:bamadapterfind	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterfind level=0	PP:bambi_3	VN:2.0.76
@PG	ID:bambi.1_3	PN:bambi	CL:/software/solexa/pkg/bambi/0.9.12/bin/bambi decode --metrics-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/24311_4.bam.tag_decode.metrics --barcode-file /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/metadata_cache_24311/lane_4.taglist --compression-level 0 -	PP:bamadapterfind_3	VN:0.9.12
@PG	ID:bamcollate2_3	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=2 level=0	PP:bambi.1_3	VN:2.0.76
@PG	ID:bwa_3	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa sampe /lustre/scratch117/core/sciops_repository/references/PhiX/Sanger-SNPs/all/bwa0_6/phix_unsnipped_short_no_N.fa /tmp/v6tWleKTPB/alnphix_bwa_aln_1_out /tmp/opf9hPyvA3/alnphix_bwa_aln_2_out /tmp/ZxTZpkxavc/alnphix_simple_cat1_out /tmp/66fH60ho6a/alnphix_simple_cat2_out	PP:bamcollate2_3	VN:0.7.16a-r1181
@PG	ID:scramble_3	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -t 2 -I sam -O bam 	PP:bwa_3	VN:1.14.9
@PG	ID:bam12auxmerge_3	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=1 zztoname=0 clipreinsert=1 /tmp/RAsOMV_HG3/simple_cat_out	PP:scramble_3	VN:2.0.76
@PG	ID:scramble.1_3	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I bam -O cram -x -3 	PP:bam12auxmerge_3	VN:1.14.9
@PG	ID:scramble.2_3	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -I cram -O bam -0 	PP:scramble.1_3	VN:1.14.9
@PG	ID:spf_3	PN:spatial_filter	CL:/software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -c  -F /dev/stdout -t /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/qc/tileviz/24311_4 --region_size 200 --region_mismatch_threshold 0.0160 --region_insertion_threshold 0.0160 --region_deletion_threshold 0.0160 /dev/stdin ; /software/solexa/pkg/pb_calibration/10.28/bin/spatial_filter -a  -f  -u  -l /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/24311_4.bam.filter.stats -F /tmp/lQkELMNsHP/tee_post_filter_creation:__APPLY_FILTER_OUT___out /dev/stdin	PP:scramble.2_3	DS:A program to apply a spatial filter	VN:v10.28-dirty
@PG	ID:samtools_3	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools split -f /nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/lane4/%!.bam -	PP:spf_3	VN:1.6
@PG	ID:bamcollate2'_3	PN:bamcollate2	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamcollate2 collate=1 level=0	PP:samtools_3	VN:2.0.76
@PG	ID:bamreset_3	PN:bamreset	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamreset resetaux=0 level=0 verbose=0	PP:bamcollate2'_3	VN:2.0.76
@PG	ID:bamadapterclip_3	PN:bamadapterclip	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamadapterclip verbose=0 level=0	PP:bamreset_3	VN:2.0.76
@PG	ID:bwa'_3	PN:bwa	CL:/software/solexa/pkg/bwa/0.7.16a/bwa mem -t 16 -p -Y -K 100000000 /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/bwa0_6/hs37d5.fa /tmp/Nd0X1H8VP2/alntgt_bamtofastq_out	PP:bamadapterclip_3	VN:0.7.16a-r1181
@PG	ID:scramble'_3	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -0 -I sam -O bam 	PP:bwa'_3	VN:1.14.9
@PG	ID:samtools'_3	PN:samtools	CL:/software/solexa/pkg/samtools/1.6/bin/samtools reheader /tmp/rgZnE8at6j/postalntgt_alterSQ_headerSQfix_out /tmp/G7njj8e0u9/postalntgt_mbuffer_headerSQfix_out	PP:scramble'_3	VN:1.6
@PG	ID:bam12split_3	PN:bam12split	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12split verbose=0 level=0	PP:samtools'_3	VN:2.0.76
@PG	ID:bamsormadup_3	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=queryname level=0	PP:bam12split_3	VN:2.0.76
@PG	ID:bam12auxmerge'_3	PN:bam12auxmerge	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/jUl1TjAWXD/amp_bamadapterclip_pre_auxmerge_out	PP:bamsormadup_3	VN:2.0.76
@PG	ID:AlignmentFilter_3	PN:AlignmentFilter	CL:uk.ac.sanger.npg.picard.AlignmentFilter INPUT_ALIGNMENT=[./initial_phix_aln_24311_4#2.bam, /tmp/eQ23isfyLi/postalntgt_bam12auxmerge_out] OUTPUT_ALIGNMENT=[/tmp/RHo7zOL2bF/alignment_filter:__PHIX_BAM_OUT___out, /dev/stdout] METRICS_FILE=24311_4#2.bam_alignment_filter_metrics.json VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=SILENT COMPRESSION_LEVEL=0 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false   	PP:bam12auxmerge'_3	DS:Give a list of SAM/BAM files with the same set of records and in the same order but aligned with different references, split reads into different files according to alignments. You have option to put unaligned reads into one of output files or a separate file	VN:V1.19
@PG	ID:bamsormadup'_3	PN:bamsormadup	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamsormadup threads=16 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane4/bsfopt_24311_4#2.tmp	PP:AlignmentFilter_3	VN:2.0.76
@PG	ID:bamstreamingmarkduplicates_3	PN:bamstreamingmarkduplicates	CL:/software/solexa/pkg/biobambam/2.0.76/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane4/bmdfopt_24311_4#2.tmp M=/nfs/sf52/ILorHSany_sf52/analysis/171110_HS36_24311_B_CBN70ANXX/Data/Intensities/BAM_basecalls_20171115-115924/no_cal/archive/lane4/24311_4#2.markdups_metrics.txt	PP:bamsormadup'_3	VN:2.0.76
@PG	ID:scramble.3_3	PN:scramble	CL:/software/solexa/pkg/scramble/1.14.9/bin/scramble -t 7 -7 -I bam -O cram -r /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa 	PP:bamstreamingmarkduplicates_3	VN:1.14.9
@PG	ID:bammerge	PN:bammerge	CL:/nfs/gs01/software/pkg/biobambam/2.0.76/bin/bammerge level=0 SO=coordinate inputformat=cram outputformat=bam irods://irods-seq-i24.internal.sanger.ac.uk/seq/24311/24311_1#2.cram irods://irods-seq-i19.internal.sanger.ac.uk/seq/24311/24311_2#2.cram irods://irods-seq-i19.internal.sanger.ac.uk/seq/24311/24311_3#2.cram irods://irods-seq-i18.internal.sanger.ac.uk/seq/24311/24311_4#2.cram	PP:scramble.3_3	VN:2.0.76
@PG	ID:bamstreamingmarkduplicates	PN:bamstreamingmarkduplicates	CL:/nfs/gs01/software/pkg/biobambam/2.0.76/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=outdata/merge_bmd_19888979.ANXX.paired158.3b752c223c.tmp M=outdata/19888979.ANXX.paired158.3b752c223c.markdups_metrics.txt resetdupflag=1	PP:bammerge	VN:2.0.76
@PG	ID:scramble	PN:scramble	PP:bamstreamingmarkduplicates	VN:1.14.9	CL:/nfs/gs01/software/pkg/staden_io_lib/1.14.9/bin/scramble -7 -I bam -O cram -r /nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa 
@CO	TY:checksum	ST:all	PA:all	HA:crc32prod	CO:2487304	BS:543946c6	NS:4ab0ce4	SQ:52e52256	ST:BC,FI,QT,RT,TC:23ed9e2a
@CO	TY:checksum	ST:all	PA:pass	HA:crc32prod	CO:2486882	BS:6fe9c22e	NS:37b020b5	SQ:1c8977ce	ST:BC,FI,QT,RT,TC:7b37224e
@CO	TY:checksum	ST:	PA:all	HA:crc32prod	CO:0	BS:1	NS:1	SQ:1	ST:BC,FI,QT,RT,TC:1
@CO	TY:checksum	ST:	PA:pass	HA:crc32prod	CO:0	BS:1	NS:1	SQ:1	ST:BC,FI,QT,RT,TC:1
@CO	TY:checksum	ST:24311_1#2	PA:all	HA:crc32prod	CO:2487304	BS:543946c6	NS:4ab0ce4	SQ:52e52256	ST:BC,FI,QT,RT,TC:23ed9e2a
@CO	TY:checksum	ST:24311_1#2	PA:pass	HA:crc32prod	CO:2486882	BS:6fe9c22e	NS:37b020b5	SQ:1c8977ce	ST:BC,FI,QT,RT,TC:7b37224e
@CO	TY:checksum	ST:all	PA:all	HA:crc32prod	CO:2466438	BS:275f9437	NS:4f872b42	SQ:7ca5d829	ST:BC,FI,QT,RT,TC:4e942f4d
@CO	TY:checksum	ST:all	PA:pass	HA:crc32prod	CO:2466044	BS:51d30498	NS:219294bb	SQ:5b1f88eb	ST:BC,FI,QT,RT,TC:6b4430ee
@CO	TY:checksum	ST:24311_2#2	PA:all	HA:crc32prod	CO:2466438	BS:275f9437	NS:4f872b42	SQ:7ca5d829	ST:BC,FI,QT,RT,TC:4e942f4d
@CO	TY:checksum	ST:24311_2#2	PA:pass	HA:crc32prod	CO:2466044	BS:51d30498	NS:219294bb	SQ:5b1f88eb	ST:BC,FI,QT,RT,TC:6b4430ee
@CO	TY:checksum	ST:all	PA:all	HA:crc32prod	CO:2470154	BS:742191a0	NS:504b021c	SQ:217089aa	ST:BC,FI,QT,RT,TC:591fe623
@CO	TY:checksum	ST:all	PA:pass	HA:crc32prod	CO:2469320	BS:715660d3	NS:6c18778a	SQ:10b80223	ST:BC,FI,QT,RT,TC:5fd7565b
@CO	TY:checksum	ST:24311_3#2	PA:all	HA:crc32prod	CO:2470154	BS:742191a0	NS:504b021c	SQ:217089aa	ST:BC,FI,QT,RT,TC:591fe623
@CO	TY:checksum	ST:24311_3#2	PA:pass	HA:crc32prod	CO:2469320	BS:715660d3	NS:6c18778a	SQ:10b80223	ST:BC,FI,QT,RT,TC:5fd7565b
@CO	TY:checksum	ST:all	PA:all	HA:crc32prod	CO:2470872	BS:1aa20f69	NS:2ae52b7e	SQ:41265670	ST:BC,FI,QT,RT,TC:7cd54b21
@CO	TY:checksum	ST:all	PA:pass	HA:crc32prod	CO:2470872	BS:1aa20f69	NS:2ae52b7e	SQ:41265670	ST:BC,FI,QT,RT,TC:7cd54b21
@CO	TY:checksum	ST:24311_4#2	PA:all	HA:crc32prod	CO:2470872	BS:1aa20f69	NS:2ae52b7e	SQ:41265670	ST:BC,FI,QT,RT,TC:7cd54b21
@CO	TY:checksum	ST:24311_4#2	PA:pass	HA:crc32prod	CO:2470872	BS:1aa20f69	NS:2ae52b7e	SQ:41265670	ST:BC,FI,QT,RT,TC:7cd54b21