FastQCFastQC Report
Sat 11 May 2024
EGAF00002423234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002423234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21207066
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTAT271387312.797022464116441TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCGCGTAT1367870.6450067161577183TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGGAT388980.18341999784411478TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTTT280050.13205504240897822TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATATCGTAT274350.12936725900697438TruSeq Adapter, Index 23 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)