FastQCFastQC Report
Mon 13 May 2024
EGAF00002423270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002423270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54814167
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC911948216.63708945900792TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG41483707.568061738491803TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC1400010.2554102482301701TruSeq Adapter, Index 8 (98% over 50bp)
CTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGC1192890.21762439626237504No Hit
GGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTTTGCAGC966580.1763376245414803No Hit
CGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTTTGCAGCTTTTGCAGACG941990.17185155801054133No Hit
CTGGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCT921860.1681791497442623No Hit
CTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTC690170.12591087993729796No Hit
GTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTT651410.11883971528747303No Hit
CAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCATCCTCTC643160.11733462993244065No Hit
GTCTTTTGCAGCTTTTGCAGACGGGCAAGAAGCTGCTTCTGCCCACACCG637320.11626921193566621No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG599200.10931480542247407TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)