FastQCFastQC Report
Wed 15 May 2024
EGAF00002423271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002423271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54814167
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC40737107.431856074726083Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG18428273.3619538540100407Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGC1178220.21494808084924466No Hit
CTGGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCT940390.17155966266895928No Hit
GGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTTTGCAGC878630.1602925024838925No Hit
CGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTTTGCAGCTTTTGCAGACG838250.15292579380071578No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG792890.14465056086686495Illumina Single End PCR Primer 1 (98% over 50bp)
CTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTC685640.1250844512514438No Hit
CAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCATCCTCTC663430.12103257904110812No Hit
GTCTTTTGCAGCTTTTGCAGACGGGCAAGAAGCTGCTTCTGCCCACACCG588430.10734998490444997No Hit
GTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTT585570.10682822198137208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)