FastQCFastQC Report
Sun 5 May 2024
EGAF00002423313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002423313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18280650
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3854032.108256544488298Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2687241.4699914937379142Illumina Single End PCR Primer 1 (100% over 50bp)
CTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGAG206450.11293362106927271No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC193430.105811336030174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)