FastQCFastQC Report
Thu 16 May 2024
EGAF00002423314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002423314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48615920
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC844098017.36258410825096TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG36205487.447247732841424TruSeq Adapter, Index 7 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1799710.37018943588849085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)