FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002424317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002424317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32142329
Sequences flagged as poor quality0
Sequence length79
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1884240.58621763220705Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT1759270.5473374378067003Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT710870.2211631895124961Illumina Paired End PCR Primer 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)