FastQCFastQC Report
Sat 13 May 2023
EGAF00002424323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002424323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30109504
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTT8601042.856586412051158Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT8384542.784682205326265Illumina Paired End PCR Primer 2 (98% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT4524741.5027613872350738Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG3574201.1870670470028333Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT3061971.016944682981161Illumina Paired End PCR Primer 2 (100% over 50bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTC656860.21815703108227888Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG540570.17953467450011795Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGACGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGG454210.1508527008614954Illumina Paired End PCR Primer 2 (100% over 24bp)
GAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT367150.12193824248981318Illumina Paired End PCR Primer 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)