FastQCFastQC Report
Sat 13 May 2023
EGAF00002424324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002424324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30109504
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10984253.6481006130157443Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT3617991.2016106276609537Illumina Single End PCR Primer 1 (100% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT554540.18417440552989514Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG533310.17712347569724163Illumina Single End PCR Primer 1 (98% over 50bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA525570.1745528587916958Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGTCTTCCGAGATCGGAAGAGCGTCGTGTAGGG456560.15163318532248155Illumina Single End PCR Primer 1 (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)