FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002424327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002424327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34123752
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT11642213.4117614030250834Illumina Paired End PCR Primer 2 (98% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTT9383772.749923279245494Illumina Paired End PCR Primer 2 (98% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT5299961.5531586327318285Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT4462641.307781160758641Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2761140.8091548666746846Illumina Paired End PCR Primer 2 (100% over 31bp)
GAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT391810.11482031635911549Illumina Paired End PCR Primer 2 (98% over 50bp)
CGGAAGACGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGG372880.10927286073348559Illumina Paired End PCR Primer 2 (100% over 24bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG364030.10667935929202627Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTTCGTATGCCGTCTT363620.10655920837778918Illumina Paired End PCR Primer 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)