FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002424328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002424328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34123752
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG14908184.368857211246876Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2785640.816334616427877Illumina Single End PCR Primer 1 (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1071950.3141360305279443Illumina Single End PCR Primer 1 (98% over 50bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA598720.17545550090740317Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGTCTTCCGAGATCGGAAGAGCGTCGTGTAGGG374360.10970657622878047Illumina Single End PCR Primer 1 (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)