FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002424329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002424329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36288274
Sequences flagged as poor quality0
Sequence length79
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT11758663.2403470057572865Illumina Paired End PCR Primer 2 (98% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTT11153963.0737091546431774Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT4125131.1367666591141812Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT3663341.0095106755421877Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2782050.7666526107028403Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG377940.10414934587409695Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGACGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGG365810.10080666829180138Illumina Paired End PCR Primer 2 (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)