FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002430682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16444403
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA6599894.013456736617316No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1353870.8233013992663643No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTG1091430.6637091051587583No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACT1052230.6398712072429751No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTT527650.32086905191997545No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCC526810.3203582398217801No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCG374280.2276032763244734No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAA312970.19032007425261957No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCAC304770.18533357519880778No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCTAGTAACA297460.18088829372522675No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG296460.18028018408451799No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGG289620.1761207141420701No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTA282720.17192475762117967No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTT278220.16918826423799027No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAAC249180.15152876027180798No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTA237840.14463279694617068No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA231310.1406618409923425No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG227310.13822940242950746No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGC213130.1296064077242573No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGG204800.12454085441715337No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGA177510.1079455423222114No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAG167640.10194350016841597No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC166140.10103133570735284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)