FastQCFastQC Report
Mon 27 Mar 2023
EGAF00002430775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56554105
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCAGCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCCTCATTTCAT1262730.22327822180193638No Hit
ACTTTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCAT1143130.20213033165320185No Hit
ACAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACC1087460.19228666071189704No Hit
ACGAGAACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACATCCTGTTAGCTTTCTTAACTAGTATT1006060.17789336423943053No Hit
ACTCGCTGAAGACTTGGAGGATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACA974000.17222445656243698No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCAT799000.14128063736487387No Hit
ACCCGGCTATGGCACCCCGAGAACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACATCCTGTTAGC671910.11880835175448361No Hit
ACCTCATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTT602290.10649801636857306No Hit
ACAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAA584100.10328162739026636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)