FastQCFastQC Report
Wed 19 Apr 2023
EGAF00002430860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71388118
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA13641591.9109048371327004No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA5544390.7766544566982422No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAG4676580.6550922101630414No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCG4546480.6368678888551172No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTA4254150.59591849724908No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGA3961830.5549705064363792No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG2594170.3633896049759989No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGGAGAA2494990.3494965366645469No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC2421310.3391754913611814No Hit
CATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTC2239350.31368665581014477No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTG2179900.3053589394246253No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGT2050750.2872676934836691No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGC2010580.28164070665093033No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAAC1665380.23328532067479354No Hit
CGATCATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGGAGAAAACGCTA1655430.2318915313049715No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACC1597260.22374311646652456No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACG1576770.22087289092002677No Hit
ATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCT1353010.18952873922240113No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCC1324880.18558830756681385No Hit
CAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCT1192490.1670432045848302No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCTAGTAACA1189570.1666341729305709No Hit
CTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCG1176710.16483275269982603No Hit
CATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCC1160670.16258588018807277No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGG1146860.16065138459035996No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG1109750.15545304051859163No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGG1076840.15084302964815519No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTG1047080.14667426867871763No Hit
GTTTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGAC1008900.1413260397199433No Hit
GCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTC987620.13834515149986165No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAA959680.13443133491766796No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGA933910.13082149048949573No Hit
AAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAA864630.12111679425419228No Hit
GAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGA849280.11896657648265781No Hit
AGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAG829470.11619160488304231No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTT779160.10914421360708794No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACATA765690.10725734498281633No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCT759500.10639025390752001No Hit
CATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGGAGAAAACGCTACCTC756390.1059546071798671No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGGAGAAAAC729670.10221168738472697No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGGAGAAAACG716070.1003066084470808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)