FastQCFastQC Report
Thu 5 Jan 2023
EGAF00002430866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42010894
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGA7400161.7614859612366258No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAG6745641.6056882769502594No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGC5214971.2413375444950066No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA4309531.0258124952066006No Hit
GGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGA3769730.8973220136662647No Hit
GGGAGAAGAACGATCATCAATGGCTGATGGCAGTTGCAGCGAAGCAACGCCAAAGAGCCGGCTTCACGCTCAGGA3374810.8033178251336428No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA2423290.5768241923154503No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG2262860.5386364784334273No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGT2196150.5227572638658915No Hit
AGAAGAACGATCATCAATGGCTGATGGCAGTTGCAGCGAAGCAACGCCAAAGAGCCGGCTTCACGCTCAGGAGAA1942830.46245861847167546No Hit
GGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAG1753900.41748694993255797No Hit
CATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCC1734300.4128214933964509No Hit
GGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG1697740.40411898875563085No Hit
GTTTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGAC1690460.40238610489936255No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGG1659060.39491185310172167No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG1624800.38675682550340396No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCC1614370.38427413613240413No Hit
GGAGAAGAACGATCATCAATGGCTGATGGCAGTTGCAGCGAAGCAACGCCAAAGAGCCGGCTTCACGCTCAGGAG1569240.3735316844245209No Hit
GGGGAGAAGAACGATCATCAATGGCTGATGGCAGTTGCAGCGAAGCAACGCCAAAGAGCCGGCTTCACGCTCAGG1550740.36912806473482807No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTA1549480.3688281425289355No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC1419330.3378480829282043No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAAC1321580.31458030862185415No Hit
GAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGA1093740.2603467567245772No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCT1064450.25337475560505807No Hit
GCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTC1034110.24615281931396177No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGATCATCAATGGCTGATGGCAGTTGCA1033290.2459576318466348No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAAC997390.2374122293136633No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGG945810.22513446155180608No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAA923150.21974062251567417No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACG887640.21128805304643122No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA867990.2066106948354872No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGT842520.20054798167351545No Hit
CATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTC787160.18737044729398047No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACT729870.17373350826573697No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGA619860.14754744328935251No Hit
AAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAA614890.14636441681055395No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC604140.14380555672059728No Hit
CCTGCATGGTTTGTCTCCCCAGTAATTGTCGACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGA594140.14142522175319572No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG568080.1352220688281473No Hit
GAGAAGAACGATCATCAATGGCTGATGGCAGTTGCAGCGAAGCAACGCCAAAGAGCCGGCTTCACGCTCAGGAGA557170.13262512337871218No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTG553010.13163490403227315No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA544890.1297020720387431No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGC544350.1295735339505034No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCC544130.1295211665812206No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCC519890.12375123462023921No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCT501280.11932143124590493No Hit
CTGCATGGTTTGTCTCCCCAGTAATTGTCGACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAG491970.11710533939125409No Hit
CAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCT491080.11689348957915535No Hit
GCATGGTTTGTCTCCCCAGTAATTGTCGACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGC489290.11646740961999047No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACC484970.115439104914073No Hit
GGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAG477710.11371098172773947No Hit
CTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCG461500.10985245874558155No Hit
ATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCTCT456450.10865038958704379No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT454040.1080767288599No Hit
ATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCC443990.10568449221766145No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCC434590.10344697734830399No Hit
CCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACG426860.10160697841850258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)