FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002430970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41844379
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGA4665111.1148713665938261No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA3802450.9087122549960653No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA3590760.8581224254755937No Hit
GGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAG2334900.5579960930953235No Hit
GGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG1862050.4449940576248007No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGT1755640.419564118755353No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGA1706780.4078875205675773No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAG1559400.3726665414248351No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG1263530.30195931453541225No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGC1125880.2690636178398059No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC932230.2227850005851443No Hit
GAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGA725180.17330404162528018No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCG698790.1669973403118254No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAAC660890.1579399708620362No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTG603240.14416273210793737No Hit
GTTTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGAC576110.13767918505852364No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCG547000.13072245617505757No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA523570.12512313780543857No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCC511880.12232945313873579No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAA493870.11802541029465391No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCGGCGAAGCAACGCCAAAAAGCCAGCTTCACGCTCAGGAGAA493840.11801824087292584No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAGAACACTGCAG472480.11291361260254333No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTA464530.11101371584460602No Hit
GTTGGGTTTTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGT462940.11063373649301857No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCC455170.10877685626544965No Hit
CATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCC443200.1059162569959516No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCTCCAGCAACTGGAAGAATCACCCCT423060.10110318520917708No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG418860.10009946616724795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)