FastQCFastQC Report
Sun 23 Apr 2023
EGAF00002430982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84011267
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2808220.3342670692015632TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2424690.2886148592426299TruSeq Adapter, Index 25 (97% over 44bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG999900.11901975005328749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)