FastQCFastQC Report
Wed 19 Apr 2023
EGAF00002430986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002430986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54245973
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2839430.5234360899010881TruSeq Adapter, Index 1 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2739700.5050513150533773TruSeq Adapter, Index 1 (100% over 63bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG720640.13284672762713648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)