FastQCFastQC Report
Sun 23 Apr 2023
EGAF00002431097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002431097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49753658
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA3967940.7975172398379231Illumina Single End PCR Primer 1 (100% over 58bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA3821240.7680319706341994Illumina Single End PCR Primer 1 (100% over 57bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG938740.18867758426928127No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGT654660.13158027496189326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)