FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002432944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002432944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961995
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1234554.167967873004512Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC681112.2994974670787762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC646002.1809624931844924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG642402.168808522634238No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC514491.7369711967778474Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC474721.6027035832268455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA457311.543925631204644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC363941.2286989005720805No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC335781.1336278420456483Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC316321.0679288790156636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA243380.8216759312557921No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG242010.8170506702408342No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA227670.7686373542156553Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC223940.7560444902844198TruSeq Adapter, Index 5 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT220470.744329413115147Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG212930.718873597018226Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC205240.6929113654817108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG204520.69048057137166TruSeq Adapter, Index 6 (100% over 49bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC176730.5966586709295593Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA172860.5835931525880361TruSeq Adapter, Index 6 (100% over 40bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC149390.5043560168062404Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC138720.4683329985364594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT113850.3843693186517871Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC106170.3584408481445782Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG104460.3526677121332075No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC104200.3517899253712447TruSeq Adapter, Index 5 (97% over 35bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC102040.34449754304109226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT92990.3139438115189256Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA92990.3139438115189256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC92390.3119181497605499No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG88420.2985150211259641Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA88350.29827869392082024TruSeq Adapter, Index 6 (100% over 38bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC80010.2701219954793982Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT79900.2697506241570293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA79850.269581819010498TruSeq Adapter, Index 6 (100% over 38bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA78560.26522664622999026Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG75280.25415302861753647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC74890.2528363484745923No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG73190.24709697349252785No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA70360.23754260219885584TruSeq Adapter, Index 6 (100% over 40bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG68910.2326472529494479Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC67760.22876473457922783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC67640.2283596022275527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG66700.22518606547276415Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC66100.22316040371438847Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC64660.21829881549428679Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT62680.21161413169164703Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC59920.20229608760311885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC59730.20165462804629988No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC59690.20151958392907485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC59100.1995276832000054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC58330.19692808394342326Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC57130.1928767604266719Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC56400.19041220528731478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC55310.1867322530929323Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA54690.1846390692759441Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC54540.18413265383635016Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC54050.18247836340034335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC53800.1816343376676868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC51690.17451076048406564Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC50610.17086456931898938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA49000.1654290436006813Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC48410.16343714287161187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA47300.15968966861861683TruSeq Adapter, Index 6 (100% over 37bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT45860.1548280803985152Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC45480.15354516128487725TruSeq Adapter, Index 5 (97% over 35bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC43490.14682671645293122Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG43180.14578012454443712Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA43030.1452737091048432No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC42750.14432840028426788No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC42270.1427078708775673Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC42200.14247154367242348No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT41780.1410535804415605Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG39970.13494283413712718No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC39950.13487531207851464Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38230.12906841503783767Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT38220.1290346540085314Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG38150.1287983268033876Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA37820.12768421283628095Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG37730.12738036357252458Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC37700.12727908048460582Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC36230.12231620917658537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC35510.11988541506653455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG35500.11985165403722829Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC35270.11907515036318427Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC35190.11880506212873418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTAGAGGGAAGCGCTTTCTGTAGATCGGAAGAGCACACGTCTGAACTC34980.11809608051330268Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGCATCCCTTTAGAGTGCAGATCGGAAGAGCACACGTCTGAACTCC34960.11802855845469017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC33570.11333577538111982TruSeq Adapter, Index 6 (100% over 36bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC33310.11245798861915701Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACAC33070.11164772391580675No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG31930.10779896657489293No Hit
TGTAAACATCCCCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC31570.10658356951986753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC31130.10509808423039202No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG30310.10232967982727857No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG29650.10010145189306531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)